Genotyping and primer details


Genotyping and primer details for 022A11 - At1g54270
Line ID 022A11
Locus At1g54270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20260812 of line 022A11 Chr1:20260812
   
Insertion allele amplicon 8474 - JA35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JA35: 5'-AGCTTTTATCTTCGATTTTGGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20260203
   
Insertion allele amplicon 8474 - JA36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JA36: 5'-CATAGATCTGCAAATTCACCAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20261389
   
wt amplicon (over InsPos) JA35 - JA36
wt amplicon start Chr1:20260203
wt amplicon end Chr1:20261389
wt amplicon size 1187 bp
Locus specific primer JA36: 5'-CATAGATCTGCAAATTCACCAGAA-3'
2nd locus specific primer JA35: 5'-AGCTTTTATCTTCGATTTTGGACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11