Sequences from confirmation amplicon


Confirmation sequences for line 022B01 and hit At3g56170 are shown.
Line ID 022B01
Locus At3g56170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20843482
Primers used for PCR MN47 - 8474, show primer details
   
Sequence Name 17-K031754-022-022-B01C-8409
GenBank Accession LN493738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K031754-022-022-B01C-8409
CCATTTACAATTGAATATATCCTGCCACAGATTTTGTATCCACCTTATTTCATTTAGAAA
ATTTCTTGATCAGCAGCAACAACATTACCATCATATCCCAAACCTTTTAAAGATTCGTAA
GTTTTCTAACCGGAAGCGTGTTAAGCTCAAACCGAACTCCTTTAGGTAAAGACACAGGAG
CTTCCGTAGGGACCTCAACGAGATTGTGAGGTGAAGGTAGTCCATAGAAAGACAAAAGTC
CCTGTCAATGCCATTAACAAGTCAACTATATCAATAATGCATCCTCCAAAACTCCTTGAC
ATTTTTGCAACTTGAAAGAACCATCATGATTAGTTAATACCTCAACATCTGCTTTTTGAT
G
   
Sequence Name 18-K031754-022-022-B01C-MN47
GenBank Accession LN494187 [GenBank]
Sequencing primer gene specific primer, MN47: 5'-CATCAAAAAGCAGATGTTGAGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K031754-022-022-B01C-MN47
AGTTGCAAATGTCAAGGAGTTTTGGAGGATGCATTATTGATATAGTTGACTTGTTAATGG
CATTGACAGGGACTTTTGTCTTTCTATGGACTACCTTCACCTCACAATCTCGTTGAGGTC
CCTACGGAAGCTCCTGTGTCTTTACCTAAAGGAGTTCGGTTTGAGCTTAACACGCTTCCG
GTTAGAAAACTTACGAATCTTTAAAAGGTTTGGGATATGATGGTAATGTTGTTGCTGCTG
ATCAAGAAATTTTCTAAATGAAATAAGGTGGATACAAAATCTGTGGCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCACTCCCAAATGGG

Last Updated on 06.12.2018 16:11