Sequences from confirmation amplicon


Confirmation sequences for line 022C12 and hit At5g06390 are shown.
Line ID 022C12
Locus At5g06390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1953215
Primers used for PCR 04eq - 8474, show primer details
   
Sequence Name 46-K110825-0022-022-C12C-04eq
GenBank Accession LN505894 [GenBank]
Sequencing primer gene specific primer, 04eq: 5'-GGATCTTACAGAGAGAGAGCTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K110825-0022-022-C12C-04eq
CTCGCCGTCATTCATCTCTTTCTCTTCTTCTCCGTCCTAATCTTCTCCGCCGCATCTGCA
TTATCCAAGAACCAGTCTCCTTCTTCCGGGTCGGGTCAGATCAACTCCAACTCTGTCCTC
GTAGCTCTTCTTGACTCTCGTTACACTGAGCTAGCTGAGCTCGTCGAGAAAGCTCTTCTC
CTTCAGACACTTGAAGACGCTGTTGGTCGTCACAATATTACCATTTTTGCTCCTCGCAAT
GAAGCTCTTGAGCAACTATACACATGTAAATGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGATTATTA
   
Sequence Name 30-K110824-0022-022-C12C-8409
GenBank Accession LN499771 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K110824-0022-022-C12C-8409
CCGGACTGAGCATTTCATGTGTATAGTTGCTCAGAGCTTCATTGCGAGGAGCAAAAATGG
TAATATTGTGACGACCAACAGCGTCTTCAAGTGTCTGAAGGAGAAGAGCTTTCTCGACGA
GCTCAGCTAGCTCAGTGTAACGAGAGTCAAGAAGAGCTACGAGGACAGAGTTGGAGTTGA
TCTGACCCGACCCGGAAGAAGGAGACTGGTTCTTGGATAATGCAGATGCGGCGGAGAAGA
TTAGGACGGAGAAGAAGAGAAAGAGATGAATGACGGCGGAGCCACCATAGATGCGGCGAT
CCATGGAGCTCTCTCTCTNTAAGATCCATG
Primers used for PCR 04eu - 8474, show primer details
   
Sequence Name 23-K110907-0022-022-C12C-04eu
GenBank Accession LN496607 [GenBank]
Sequencing primer gene specific primer, 04eu: 5'-CACAACATAGACAACACAACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K110907-0022-022-C12C-04eu
TTCACTTGCCTCGCCGACACTTCAAGAAAATTAAAGAAAAGAAAGTAAACTCTTTCACCA
AAAAAAATAAAGAAAATTTATTGTTCCATCTTTCTGATAATCGGATCTTACAGAGAGAGA
GCTCCATGGATCGCCGCATCTATGGTGGCTCCGCCGTCATTCATCTCTTTCTCTTCTTCT
CCGTCCTAATCTTCTCCGCCGCATCTGCATTATCCAAGAACCAGTCTCCTTCTTCCGGGT
CGGGTCAGATCAACTCCAACTCTGTCCTCGTAGCTCTTCTTGACTCTCGTTACACTGAGC
TAGCTGAGCTCGTCGAGAAAGCTCTTCTCCTTCAGACACTTGAAGACGCTGTTGGTCGTC
ACAATATTACCATTTTTGCTCCTCGCAATGAAGCTCTTGAGCAACTATACACATGTAAAT
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 07-K110898-0022-022-C12C-8409
GenBank Accession LN489329 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K110898-0022-022-C12C-8409
AGTTGCTCAGAGCTTCATTGCGAGGAGCAAAAATCGGTAATATTGTGACGACCAACAGCG
TCTTCAAGTGTCTGAAGGAGAAGAGCTTTCTCGACGAGCTCAGCTAGCTCAGTGTAACGA
GAGTCAAGAAGAGCTACGAGGACAGAGTTGGAGTTGATCTGACCCGACCCGGAAGAAGGA
GACTGGTTCTTGGATAATGCAGATGCGGCGGAGAAGATTAGGACGGAGAAGAAGAGAAAG
AGATGAATGACGGCGGAGCCACCATAGATGCGGCGATCCATGGAGCTCTCTCTCTGTAAG
ATCCGATTATCAGAAAGATGGAACAATAAATTTTCTTTATTTTTTTTGGTGAAAGAGTTT
ACTTTCTTTTCTTTAATTTTTCTTGAAGTGTCGGCGATGGCAAGTGGAAGTGTTAAAGAA
AAAGAGATGAGTTTGGTTGTGTTGCTAATGTTGTG

Last Updated on 06.12.2018 16:11