Sequences from confirmation amplicon


Confirmation sequences for line 022E02 and hit At1g49730 are shown.
Line ID 022E02
Locus At1g49730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18402689
Primers used for PCR 02hw - 8474, show primer details
   
Sequence Name 33-K112689-0022-022-E02C-02hw
GenBank Accession LN501061 [GenBank]
Sequencing primer gene specific primer, 02hw: 5'-GTCTAGAGATGGCTCTGTTTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K112689-0022-022-E02C-02hw
GTTTGGGAGAGATACTGAACAGAAAACTTGACTACTTTTGATGGAAGCTGAGTTTTAAAA
CTTGAAGTTTTTTCAGGTTATGTAGATCCGGAATATGTAGTAACGCAAGAGTTGACAGAG
AAGAGTGATGTGTACAGCTACGGAGTTGTGTTGCTGGAGCTCATAACCGGGAGAAGAGCA
GTTGATGAAGGTAGGAACCTTGTGGAAATGTCTCAGCGGTTTTTGTTGGCGAAATCAAAA
CACCTCGAGTTGGTAGACCCGAGAATCAAAGACTCTATTAATGATGCTGGAGGAAAACAA
CTTGATGCAGTTGTGACGGTTGTGAGATTGTGTACCGAGAAAGAAGGCCGGTCTAGGCCA
TCAATTAAGCAAGTCTTGAGGCTGTTGTGTGAAAGCTGTGATCCGGTGCATAGCGCATTT
GCTAAAGCGGTTGAAGAAGAGATAGGCTGGGATAGTAGGAAGAGAAGCAATTTGAGAATT
CAAAGAGGTGATTCCCGGATCTTTGGTCCATCGTCTAGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 01-K112688-0022-022-E02C-8409
GenBank Accession LN486644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112688-0022-022-E02C-8409
CAAAGATCCGGGGAATCACCTCTTTGAATTCTCAAATTGCTTCTCTTCCTACTATCCCAG
CCTATCTCTTCTTCAACCGCTTTAGCAAATGCGCTATGCACCGGATCACAGCTTTCACAC
AACAGCCTCAAGACTTGCTTAATTGATGGCCTAGACCGGCCTTCTTTCTCGGTACACAAT
CTCACAACCGTCACAACTGCATCAAGTTGTTTTCCTCCAGCATCATTAATAGAGTCTTTG
ATTCTCGGGTCTACCAACTCGAGGTGTTTTGATTTCGCCAACAAAAACCGCTGAGACATT
TCCACAAGGTTCCTACCTTCATCAACTGCTCTTCTCCCGGTTATGAGCTCCAGCAACACA
ACTCCGTAGCTGTACACATCACTCTTCTCTGTCAACTCTTGCGTTACTACATATTCCGGA
TCTACATAACCTGAAAAAACTTCAAGTTTTTAAAACTCAGCTTCCATCAAAAGTAGTCAA
GTTATTCTGTTCAGTATCTCTCCCAAACCTGGAGTTCCACGGATATCGGTATTCACAGGC
TCAAAGCAAACAG
Primers used for PCR 51lf - 8474, show primer details
   
Sequence Name 76-K113840-0022-022-E02C-51lf
GenBank Accession LN513437 [GenBank]
Sequencing primer gene specific primer, 51lf: 5'-ACTACTTTTGATGGAAGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113840-0022-022-E02C-51lf
ATCCGGAATATGTAGTAACGCAAGAGTTGACAGAGAAGAGTGATGTGTACAGCTACGGAG
TTGTGTTGCTGGAGCTCATAACCGGGAGAAGAGCAGTTGATGAAGGTAGGAACCTTGTGG
AAATGTCTCAGCGGTTTTTGTTGGCGAAATCAAAACACCTCGAGTTGGTAGACCCGAGAA
TCAAAGACTCTATTAATGATGCTGGAGGAAAACAACTTGATGCAGTTGTGACGGTTGTGA
GATTGTGTACCGAGAAAGAAGGCCGGTCTAGGCCATCAATTAAGCAAGTCTTGAGGCTGT
TGTGTGAAAGCTGTGATCCGGTGCATAGCGCATTTGCTAAAGCGGTTGAAGAAGAGATAG
GCTGGGATAGTAGGAAGAGAAGCAATTTGAGAATTCAAAGAGGTGATTCCCGGATCTTTG
GTCCATCGTCTAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATATCCGCACGGNTTATT
   
Sequence Name 68-K113841-0022-022-E02C-8409
GenBank Accession LN512056 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113841-0022-022-E02C-8409
ACGATGGACCAAAGATCCGGGAATCACCTCTTTGAATTCTCAAATTGCTTCTCTTCCTAC
TATCCCAGCCTATCTCTTCTTCAACCGCTTTAGCAAATGCGCTATGCACCGGATCACAGC
TTTCACACAACAGCCTCAAGACTTGCTTAATTGATGGCCTAGACCGGCCTTCTTTCTCGG
TACACAATCTCACAACCGTCACAACTGCATCAAGTTGTTTTCCTCCAGCATCATTAATAG
AGTCTTTGATTCTCGGGTCTACCAACTCGAGGTGTTTTGATTTCGCCAACAAAAACCGCT
GAGACATTTCCACAAGGTTCCTACCTTCATCAACTGCTCTTCTCCCGGTTATGAGCTCCA
GCAACACAACTCCGTAGCTGTACACATCACTCTTCTCTGTCAACTCTTGCGTTACTACAT
ATTCCGGATCTACATAACCTGAAAAAACTTCAAGTTTTTAAAACTCAGCTTCCATAANAA
GTAG

Last Updated on 06.12.2018 16:11