Sequences from confirmation amplicon


Confirmation sequences for line 026F07 and hit At2g46290 are shown.
Line ID 026F07
Locus At2g46290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19007391
Primers used for PCR 02po - 3144, show primer details
   
Sequence Name 78-K115121-0022-026-F07C-02po
GenBank Accession LN513727 [GenBank]
Sequencing primer gene specific primer, 02po: 5'-AGAATTGACAACTTACGGTCTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K115121-0022-026-F07C-02po
AGGAAGTTCCCACGAAGTGATCAGTGGTAATCACTGCAAGATGATCACCAACAGAGAAAT
CCACAGACCTTGCAGGGGCACCAAACTTGAAAGTGAACAATTCTTTGCCAGATTTCACAT
CCCACAGCTTTGCAGTCTGATCAGCACTACCAGTGATCAATCTAGACGAGTCTCCTAAGA
AATCACAAACAAAACACTAATCGTTAAGCTATGAAAGACTAAAACAACAAAGACAACGAC
TTAAATCCTAAACTGAATCCATTCCATCCACTATATACTATTTGAATTTGAGAGGCTGAG
ACCACTTACTGGAGATATCACATCAATCCA
   
Sequence Name 30-K115120-0022-026-F07C-3144
GenBank Accession LN499845 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K115120-0022-026-F07C-3144
TGTGGATGGAATGGATTCAGTTTAGGATTTAAGTCGTTGTCTTTGTTGTTTTAGTCTTTC
ATAGCTCAACGATTAGTGTTTTGTTTGTGATTTCTTAGGAGACTCGTCTAGATTGATCAC
TGGTAGTGCTGATCAGACTGCAAAGCTGTGGGATGTGAAATCTGGCAAAGAATTGTTCAC
TTTCAAGTTTGGTGCCCCTGCAAGGTCTGTGGATTTCTCTGTTGGTGATCATCTTGCAGT
GATTACCACTGATCACTTCGTGGGAACTTCCTCTGCTATTCATGTCAAACGCATTGCAGA
AGATCCCGAAGACCGTAAGTTGTCAATTC

Last Updated on 06.12.2018 16:11