Sequences from confirmation amplicon


Confirmation sequences for line 026G04 and hit At3g45600 are shown.
Line ID 026G04
Locus At3g45600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16734585
Primers used for PCR EO02 - 8474, show primer details
   
Sequence Name 53-K018493-022-026-G04C-EO02
GenBank Accession LN507996 [GenBank]
Sequencing primer gene specific primer, EO02: 5'-ACAATTTAGGTCCTCCATGTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K018493-022-026-G04C-EO02
CCATATGACGAGGGAGACTCAATTATTGAAAAGATAATTATTATCTTTTTTTTTCCAAGT
GAAGAAACTTTGTGTTAATTTTTTATTAATTAACTTAGTTGACTATGAGTATTGGAGTAT
AGATCTGATTTAGTTTTGCCTCTAGCTTACTCCATCAAAAAAAAAAAAAGATAATAAAGG
GGAAAAAAATTATTTTATTAGTTTATTTGACCGGATGGATGGATGGTGGGGAAAAACCCA
ATGAAACATTTTTAAGCAGGAAAAAAGGGTTTGATTCCTGGAAAAAGGATTCTAAGCTTC
AATTCTAATTGGCCAAAAATATTTCGGGCCTTGGCAGTTTTGTTCTCAAATGTAAAAATC
TTTAATTAGTTTTGGGAGCGCCCCACAAAAGTATACATTTTTTGGGACCCAAAAAATTAT
TTCTTGGGGGAATTTTGGAAATTTTTCTATAAAAGAAAACTTATTTTTTTTCAAATTCCC
AGTTTTACCCGGGGGTAACCAAAAATTTAATAAAAATGCGGGGATTTGGAAGGGGAACGG
GAA
   
Sequence Name 54-K018493-022-026-G04C-8409
GenBank Accession LN508331 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K018493-022-026-G04C-8409
GATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTCCCGTT
CAAGATCCATGCATCTTCATCATATTTTTGGTTACTCACGTGTAAAGCTGGGAATTTGAA
CATATATATGTCTACTTTAATAGAAAAATTTCAAAAATACAACAAAAAAATAATTATTTG
GGTCCAAAAAATTGTATACTTTTGGGGGCCCCTCCAAAAGCTAATAATAAATTTTAACAT
ATAAAAACAAAACTGCAAAGGCCAAAAATATTTCTGGCAAATAAAAATCGAACCTAAAAA
TCATTGTTCAAGGAATCATACCTTTTTTACATGCTAAAAATTGTTTCATTGGCTTCAACA
CAACAATCAATCAATCAGGTCAAATAAACTAATAAAATAATTTTTCTACATTTTATTATC
TTTTTTTTTTTTGGAGGGAGAAAGCTAAAGGCAAAACTAAATCAAATTTTTCCCCCAATA
CCCAAAGTCACCTAAGTTATTTAAAAAAAATTTAACCCAAGGTTTCTTCTTTTGGAAAAA
AAAAAAAAAAAATTATTTTTCCAAAAATGGAGCCCCCCCCGCCAAAGG
Primers used for PCR FL47 - 3144, show primer details
   
Sequence Name 84-K028756-022-026-G04C-3144
GenBank Accession LN514371 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K028756-022-026-G04C-3144
TTCATTTTCATTTGGAGAGGAACGGGTACCCGGGGAATCAGAATTGTCGTTTCCCGCCTT
CGGTTTAAACTATCAGTGTTTGTAGGAGCTTCCTTCGGTATAAACTTCGCAAGAACTTCC
CACTATCCCACTAAAGTGTAGTTGTTCAAGTTCTTGTTTTGTCATAAATCTTGAATCTAG
AAATGCTCTGTTTTGTTTATTTTCTTTATGAAAGATTCTTTTGCTATATATATTTTGGTG
TTGATTATTATATTTATGAGCCTAATAATCTAATATTGTGGTTGTAATAATTGTTACTTA
GAGTGTTCACTGTTTAGTGTCTCTCTTCTTTACCTGCTTATGATAAAAGAGTTATTAATA
TTGCGGTTTATGATATGTAATTGTTGTTGTTAATTTGCAGTCCGGTTGTTGCAAGCCACC
AACAGATTGCGGTTTTTCATATGTGAATGAGACCGGATGGGACACGAGAGGAGGGATGAT
AGGACCGAACCAGGACTGTATGGTGTGGAGCAACGACCAGAGCATGCTCTGTTATCAGTG
TAGTTCTTGTAAAGCTGGTGTTCTTGGGAGTTTGAAGAAGAGTTGGAGA
   
Sequence Name 85-K028756-022-026-G04C-FL47
GenBank Accession LN514488 [GenBank]
Sequencing primer gene specific primer, FL47: 5'-TCACCGATACTTTTCTCCAACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K028756-022-026-G04C-FL47
CACACCATACAGNTCCTGAGTTCGGTCCTATCATCCCTCCTCTCGTGTCCCATCCGAGTC
TCATTCACATATGAAAAACCGCAATCTGTTGGTGGCTTGCAACAACCGGACTGCAAATTA
ACAACAACAATTACATATCATAAACCGCAATATTAATAACTCTTTTATCATAAGCAGGTA
AAGAAGAGAGACACTAAACAGTGAACACTCTAAGTAACAATTATTACAACCACAATATTA
GATTATTAGGCTCATAAATATAATAATCAACACCAAAATATATATAGCAAAAGAATCTTT
CATAAAGAAAATAAACAAAACAGAGCATTTCTAGATTCAAGATTTATGACAAAACAAGAA
CTTGAACAACTACACTTTAGTGGGATAGTGGGAAGTTCTTGCGAAGTTTATACCGAAGGA
AGCTCCTACAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGG
TACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGA
TAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTT
GAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTG

Last Updated on 06.12.2018 16:11