Sequences from confirmation amplicon


Confirmation sequences for line 028F05 and hit At5g39450 are shown.
Line ID 028F05
Locus At5g39450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15786968
Primers used for PCR 09by - 8474, show primer details
   
Sequence Name 23-K107979-0022-028-F05C-09by
GenBank Accession LN496586 [GenBank]
Sequencing primer gene specific primer, 09by: 5'-ATCTTCTGTACATTTTCCTGGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K107979-0022-028-F05C-09by
TACCGGCATACTCATCGTAGCGATGGGATCCTTAATCGGTAGCTTATAAAGGGCATAACG
GGTTTCGTCCATGTTTGGCCAACCTTGGTAAGAAGACAACTTAATATTGGAAGCTTTGAG
ACTAAGACCTATTGAATCACCAAAATTAAGAAACTTTTGGAGATTCAAAGGTCTAGTCTC
ACTTCTTGAAGAAGACATGTTCTTTGAAGAACTAGACCTCCCGAGAATATGCTTTATGCC
ACTTCTGAAATACCTACTAAAGCTCTTCCTTTGTGTCTGATTCTCAGCGTCCATGTTGCG
AATATCGCCTTCAAAACCTAGATGCTTCCACGATTTGTTTATGTCTACCTGATTTGGAAT
ATTTGTGCACTCATCCTCCAGGTTCATGTGATTCCAATTATAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 07-K107978-0022-028-F05C-8409
GenBank Accession LN489304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K107978-0022-028-F05C-8409
TTGGAATCCATGAACCTGGAGGATGAGTGCACAATATTCCACATTAGGTAGACATAAACA
AATCGTGGAAGCATCTAGGTTTTGAAGGCGATATTCGCAACATGGACGCTGAGAATCAGA
CACAAAGGAAGAGCTTTAGTAGGTATTTCAGAAGTGGCATAAAGCATATTCTCGGGAGGT
CTAGTTCTTCAAAGAACATGTCTTCTTCAAGAAGTGAGACTAGACCTTTGAATCTCCAAA
AGTTTCTTAATTTTGGTGATTCAATAGGTCTTAGTCTCAAAGCTTCCAATATTAAGTTGT
CTTCTTACCAAGGTTGGCCAAACATGGACGAAACCCGTTATGCCCTTTATAAGCTACCGA
TTAAGGATCCCATCGCTAACGATGAGTATGCCGGGTTATGGGGAGGAACTTTTGGCTGGC
CACCAGGAAAATGTACAGAAGA

Last Updated on 06.12.2018 16:11