Sequences from confirmation amplicon


Confirmation sequences for line 028G04 and hit At5g60010 are shown.
Line ID 028G04
Locus At5g60010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24160825
Primers used for PCR TH22 - 8474, show primer details
   
Sequence Name 37-K103676-0022-028-G04C-TH22
GenBank Accession LN502587 [GenBank]
Sequencing primer gene specific primer, TH22: 5'-TAACACTCCTACGGAGGATTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103676-0022-028-G04C-TH22
GATTCATGGGTGAAGCTCCTCAAAGAACCCGAAATTAACCTCAACAAGAACGAAGGTGGT
CTGAAGAAGAACGCGTCAAGAAATCTCGGCGTGGGATCGATAATCCGAACATTGAGCGTT
TCAAACTGGAGAAAGAGCGGGAATCTAGGCTCTCCGAGCACAAGGAAAAGTGGAAACCTG
GGGCCTCCGACCAATGCCGTGCCCAAGAAGACAGGTCCACAAAGAGTGGAGAGGACAACA
TCAAGCGCGGCTAGGGGACTTCAGAGCTTACGGTTCCTTGATCGGACAGTCACCGGACGA
GAACGAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATT
   
Sequence Name 53-K103677-0022-028-G04C-8409
GenBank Accession LN508127 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103677-0022-028-G04C-8409
CTCGTTCTCGTCCGGTGACTGTCCGATCAAGGAACCGTAAGCTCTGAAGTCCCCTAGCCG
CGCTTGATGTTGTCCTCTCCACTCTTTGTGGACCTGTCTTCTTGGGCACGGCATTGGTCG
GAGGCCCCAGGTTTCCACTTTTCCTTGTGCTCGGAGAGCCTAGATTCCCGCTCTTTCTCC
AGTTTGAAACGCTCAATGTTCGGATTATCGATCCCACGCCGAGATTTCTTGACGCGTTCT
TCTTCAGACCACCTTCGTTCTTGTTGAGGTTAATTTCGGGTTCTTTTGAGGAGCTTTCAC
CCATTGAATCGATCTCTACACTCTCTAACATCCATTTTGTTGAATCCTCCGTAGGAGTGT
TA

Last Updated on 06.12.2018 16:11