Sequences from confirmation amplicon


Confirmation sequences for line 029B02 and hit At2g46500 are shown.
Line ID 029B02
Locus At2g46500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19088255
Primers used for PCR PC19 - 8474, show primer details
   
Sequence Name 16-K103735-0022-029-B02C-PC19
GenBank Accession LN493402 [GenBank]
Sequencing primer gene specific primer, PC19: 5'-AAGAATCCGCTCTCTTTCTCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K103735-0022-029-B02C-PC19
CGGCGACTCATTGGCTCGATTTGATTCACCTGTAGCTATCAATCAATCCCTGATCTGTTT
AGGTTTGATCTCCTCTGTTTTTTCCTAGGGCTTGTTATTGTTGTTTCTCGAAAAAGAAGA
AGAAGCGATGGTATTGGGATTTTAGGGATTATTGCGGTTTGTTGTATATATATATATAGA
AGCTGAGGGATTTTGTGTTGTGAAGAACATCGAATCCTGTTTTTTTCCGTAAGAAAGTTC
TTAGATTTCTCCAAGTGAAAGAAGATGTCATCAGCTGGTGTTGCGTTAAGTCCGGTTCGT
AGCGAGCCATTGATTATGCCTCTGGTTCGTGCCAATTCTTGTCTTGATTCTTATCCTGAC
GACACCATCATGATTTACCTTACACTTCCTGGATCGGTGATACCAATGCGAGTGCTTGAG
TCTGATTCCATTGAGTCTGTTAAGCTTCGGATTCAGTCTTACCGCGGCTTTGTTGTGAGG
AATCAGAAGCTTGTTTTCGGTGGTCGAGAGCTAGCTAGAAGCAATTCTAATATGCGTGAT
TATGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGC
   
Sequence Name 32-K103736-0022-029-B02C-8409
GenBank Accession LN500553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K103736-0022-029-B02C-8409
CGGACTGAGCCTTTACATTGAATAATCCTGATCATAATCACGCATATTAGAATTGCTTCT
AGCTAGCTCTCGACCACCGAAAACAAGCTTCTGATTCCTCACAACAAAGCCGCGGTAAGA
CTGAATCCGAAGCTTAACAGACTCAATGGAATCAGACTCAAGCACTCGCATTGGTATCAC
CGATCCAGGAAGTGTAAGGTAAATCATGATGGTGTCGTCAGGATAAGAATCAAGACAAGA
ATTGGCACGAACCAGAGGCATAATCAATGGCTCGCTACGAACCGGACTTAACGCAACACC
AGCTGATGACATCTTCTTTCACTTGGAGAAATCTAAGAACTTTCTTACGGAAAAAAACAG
GATTCGATGTTCTTCACAACACAAAATCCCTCAGCTTCTATATATATATATACAACAAAC
CGCAATAATCCCTAAAATCCCAATACCATCGCTTCTTCTTCTTTTTCGAGAAACAACAAT
AACAAGCCCTAGGAAAAAACAGAGGAGATCAAACCTAAACAGATCAGGGATTGATTGATA
GCTACAGGTGAATCAAATCGAGCCAATTGATGTCGCCGATGACGTACCATATGAAGAAAA
AAGAAAGAGAGAAA

Last Updated on 06.12.2018 16:11