Sequences from confirmation amplicon


Confirmation sequences for line 029E10 and hit At1g09580 are shown.
Line ID 029E10
Locus At1g09580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3105534
Primers used for PCR 07mm - 8474, show primer details
   
Sequence Name 32-K114362-0022-029-E10C-07mm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 07mm: 5'-TGCTCAGATGCTTGTGTATAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K114362-0022-029-E10C-07mm
AAATAAAAAAAATTGAACCTTTAGGGGGCCTTTAAAGGAAGCCGGGGTTTTGAAGGGGAA
GGTTGGGAAACAAGGATAATTATACCAAACAAGGGATTTCCTTTTATGGACCAAGTACCA
TTGGGTATTAAGAAACCATGGCTATGTGCAATTCCCAAATAGTTGGAGGGATTGTTAAAA
ATAGGGCCCTTGGAATTGAAACCATTCTTTAAATTCAACTTGAAAAAAGGAAAACAAGGT
TCCTAAAGGGAAACCCCTGTCCCTTTTAATCCTGGGCCCTAATTTTTAAAAACCATTATG
GTGCAGGTAACACCCCCAAATGGCAACAATCTGCACAATAGGAAAAACTTACCCCATTTA
CAATTTCCAATATGGCTCCAGGTCCGGAAAATCTCCTGGGATCACCATTGAGTATAAGGG
TCAATATGGAAAAAAAAAAAAAGTATTTCCCATTTTTTTTCCATTTCAAAAATGTAAATT
CCCC
   
Sequence Name 02-K114361-0022-029-E10C-8409
GenBank Accession LN487131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114361-0022-029-E10C-8409
GTAATTGCGTTACGTTTTCCATATTGTGCAGATTGTTGCCATATGGGGATGTCACCTGCA
CCATAATGATTCTCAAAACTCAGAGCCAAGAATAAAAAGGAACAGAGGTTACCATTCAGA
AACATTGTATTCCTTTTTTCAAGTCGACTTCAAAGACTGCTCTCAATCCAAAGGACACTA
TCTTAAACAATCCATCAAACTATCTGGAACTAGCACATAGCAATGATTACTAAATACCAA
ATGATACTTGCTCAATAAAAGAAACTCCCTTGTTCGTAATAATTATCATTGTTCCCAAAC
ATACACATCAAAAACACAGACTTCATTAAAAGACCACATAAAGTTTCAATTTTTCTTATC
TCAATCTCACAGCTAATTACTGATATTACGGCAAAAAAAAAAGGGTATTCACGGGGATAA
ACCTTTACCCAACCTTCTAA

Last Updated on 06.12.2018 16:11