Sequences from confirmation amplicon


Confirmation sequences for line 029H12 and hit At4g27730 are shown.
Line ID 029H12
Locus At4g27730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13835785
Primers used for PCR KS41 - 8474, show primer details
   
Sequence Name 70-K033097-022-029-H12C-8409
GenBank Accession LN512315 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K033097-022-029-H12C-8409
CATTTACATTGAATATATCCTCTTGATTTCTTTTCTTTCTCGTGTAACGCACCAAAGAGA
GAGACTTGAACAAGATTGCTTGGCCACCACATTTCACCAGGCTCAACAAGGTGTTTCCTG
AAGAGACCAGCCCAACCAAATCCAAGAATCTAAACACAAGAAACGATTTCAAGGTTCAAT
AAACTGCAAAACAAGAAACAGAGGATAAAGTGAAGTGAGAGTACCTGAGTGGTGATCATG
ACGAGAAATGCAGGGAGGAAAGGAAGAGAACGTTTGTAGTAAAGCTTAATAGCACTAAGT
ATGTGAGTAGCGTAAACAGAGCCGGCGCCGGAATTTGCAAAGATAGTTATCAGAACATGT
TCCTTGACGTTGAAGGCACCCGGGTTTAAGGTAAACTGAAACCTCGTACCTTCCAAGAAT
CTTTTTGTCGGAAGCACTCTCGCCATGAGATGACCAAGCGGCACGACGGCGATCTGAGCG
GAGATTCCGGTGATACTCAACGGCATCGTTCTGTACCAGAAAAACTGGTTGATAAAGGA
   
Sequence Name 22-K033097-022-029-H12C-KS41
GenBank Accession LN496011 [GenBank]
Sequencing primer gene specific primer, KS41: 5'-CGTGCATTGTACTCTCCTTTATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K033097-022-029-H12C-KS41
GTTGAGTATCACCGGAATCTCCGCTCAGATCGCCGTCGTGCCGCTTGGTCATCTCATGGC
GAGAGTGCTTCCGACAAAAAGATTCTTGGAAGGTACGAGGTTTCAGTTTACCTTAAACCC
GGGTGCCTTCAACGTCAAGGAACATGTTCTGATAACTATCTTTGCAAATTCCGGCGCCGG
CTCTGTTTACGCTACTCACATACTTAGTGCTATTAAGCTTTACTACAAACGTTCTCTTCC
TTTCCTCCCTGCATTTCTCGTCATGATCACCACTCAGGTACTCTCACTTCACTTTATCCT
CTGTTTCTTGTTTTGCAGTTTATTGAACCTTGAAATCGTTTCTTGTGTTTAGATTCTTGG
ATTTGGTTGGGCTGGTCTCTTCAGGAAACACCTTGTTGAGCCTGGTGAAATGTGGTGGCC
AAGCAATCTTGTTCAAGTCTCTCTCTTTGGTGCGTTACACGAGAAAGAAAAGAAATCAAG
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA

Last Updated on 06.12.2018 16:11