Sequences from confirmation amplicon


Confirmation sequences for line 030B06 and hit At4g12350 are shown.
Line ID 030B06
Locus At4g12350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7325682
Primers used for PCR GG24 - 8474, show primer details
   
Sequence Name 76-K100900-0022-030-B06C-8409
GenBank Accession LN513354 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K100900-0022-030-B06C-8409
CCATTTACAATTGAATATATCCTATAATTGAAAATTAATCCATAATATTTTAATATAAGA
TAATGTATGGATCATTTGAGACTGAATAGTTTTATAAGAAAACGATATATCTTAATTCAG
TTTCCTTTCTTTGAATTAAAGTCTTCTAAATATTTGTATAAAAAAGTTGTACATCTTATT
TCAACTTCGAGACAAAGTAACGTTCCTCGTTTCCAAATAGAATATCATAGCGTATATATA
TTGTCAACTTTCTTGTGTTAGATTCCAAGACACAAGACTTTGACATCTCCACCAACACCT
AGCTCAAATCATTATTTTTCAAAGGTAAACATATTCAATGTAACTAAACATATTTCTTAA
AATTCTTTTTATGTATATATAGATGGTCCAAGATCGCTGCAAGATTACCAG
   
Sequence Name 76-K100906-0022-030-B06C-GG24
GenBank Accession LN513355 [GenBank]
Sequencing primer gene specific primer, GG24: 5'-GTCTGTTCTTCCTGGTAATCTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K100906-0022-030-B06C-GG24
GAATTTTAAGAAATATGTTTAGTTACATTGAATATGTTTACCTTTGAAAAATAATGATTT
GAGCTAGGTGTTGGTGGAGATGTCAAAGTCTTGTGTCTTGGAATCTAACACAAGAAAGTT
GACAATATATATACGCTATGATATTCTATTTGGAAACGAGGAACGTTACTTTGTCTCGAA
GTTGAAATAAGATGTACAACTTTTTTATACAAATATTTAGAAGACTTTAATTCAAAGAAA
GGAAACTGAATTAAGATATATCGTTTTCTTATAAAACTATTCAGTCTCAAATGATCCATA
CATTATCTTATATTAAAATATTATGGATTAATTTTCAATTATAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11