Sequences from confirmation amplicon


Confirmation sequences for line 030C12 and hit At1g11260 are shown.
Line ID 030C12
Locus At1g11260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3777294
Primers used for PCR KQ01 - 8474, show primer details
   
Sequence Name 49-K034240-022-030-C12C-8409
GenBank Accession LN506751 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K034240-022-030-C12C-8409
AAAGCCATTTACAATTGAATATATCCTGATCTATATAACTCTGATAAATAATCTCTGATC
TCTCCATCTAATATCTCTTTCTCAATTTCGGTTCCACTTTCCTTTCGTTTGCAAAAACCC
ATCCCATCAAAAATAAACAAGAGGGCCTAAAGAAGAATCCTAAAGACTTTACGGGTCTTG
TTTAGGATAAAAGAAATGCCTGCCGGTGGATTCGTCGTCGGGGATGGCCAAAAGGCTTAT
CCCGGCAAACTCACTCCCTTTGTTCTCTTCACTTGCGTTGTTGCTGCCATGGGCGGTCTC
ATCTTCGGATACGATATCGGAATCTCCGGTTAGTGTCTCTCTATTTTTCAGATTTTACAT
TTCCACCCCAAAAGTATCTATGTAGTCATCAACACACCCAAATCAGAAATAATAATTGTC
GTTTTTGATACGAAAACATTAGAGTACCCACCCCATATTTTCACTTTAGTCATGATGACA
CACAAGGCTCAAATTTACAGA
   
Sequence Name 50-K034240-022-030-C12C-KQ01
GenBank Accession LN507084 [GenBank]
Sequencing primer gene specific primer, KQ01: 5'-TTCTGTAATTTTGAGCCTTGTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K034240-022-030-C12C-KQ01
GGGTGGGTACTCTAATGTTTTCGTATCAAAAACGACAATTATTATTTCTGATTTGGGTGT
GTTGATGACTACATAGATACTTTTGGGGTGGAAATGTAAAATCTGAAAAATAGAGAGACA
CTAACCGGAGATTCCGATATCGTATCCGAAGATGAGACCGCCCATGGCAGCAACAACGCA
AGTGAAGAGAACAAAGGGAGTGAGTTTGCCGGGATAAGCCTTTTGGCCATCCCCGACGAC
GAATCCACCGGCAGGCATTTCTTTTATCCTAAACAAGACCCGTAAAGTCTTTAGGATTCT
TCTTTAGGCCCTCTTGTTTATTTTTGATGGGATGGGTTTTTGCAAACGAAAGGAAAGTGG
AACCGAAATTGAGAAAGAGATATTAGATGGAGAGATCAGAGATTATTTATCAGAGTTATA
TAGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11