Sequences from confirmation amplicon


Confirmation sequences for line 030D12 and hit At5g57220 are shown.
Line ID 030D12
Locus At5g57220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23189279
Primers used for PCR 05co - 8474, show primer details
   
Sequence Name 29-K108475-0022-030-D12C-05co
GenBank Accession LN499323 [GenBank]
Sequencing primer gene specific primer, 05co: 5'-GTTCATAACAAGGAAGAAGCGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K108475-0022-030-D12C-05co
CATGACAACAGTGGTGCGAGCCATCCAGGAGATTATCTACCGATTCTTAAAGTGTTCGGA
CACGGCTACGAGAAAAAAGTGAAAGCACTAGGCGAAGCCATGGACGCTTTCTTACAACGA
CTGCTCGATGAATGTCGAATAAATGGAGAGAGCAACACAATGGTTAGTCACTTGTTGTCT
TTACAACTAGACCAACCCAAGTATTACAGTGACGTCATCATCAAAGGGCTCATGCTCGTA
AGTATTCTCATACATATAAACCTTAAATATAATTTTTTTTCCTTGGACTCACTAAATGCA
AACATAAAACTGAAAGAAGAAAGATGGTTTATGTTTTTACATTTATATTATAAAGATATT
ATATGTGTACATGTGTAGATGAACTAACAACTTGTGTGTGAATTTAATGTATATAGAGTA
TGATGCTAGCTGGAACGGATACTGCGGCCGTAACATTGGAATGGGCGATGGCAAATTTAT
TAAAAAAACCTGAAGTGTTGAAAAAGGCAAAAGCTGAGATAGACGAGAAGATTGGAGAAG
AGAGATTGGTCGATGAACCTGACATTGCGAATCTACCTTATCTCCAAAACATAGTTTCCG
AGACTTTTCGGTTGTGTCCATTATATAAATCTACATGGCATCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 13-K108474-0022-030-D12C-8409
GenBank Accession LN492074 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K108474-0022-030-D12C-8409
AGATTTATATAATGGACACAACCGAAAAGTCTCGGAAACTATGTTTTGGAGATAAGGTAG
ATTCGCAATGTCAGGTTCATCGACCAATCTCTCTTCTCCAATCTTCTCGTCTATCTCAGC
TTTTGCCTTTTTCAACACTTCAGGTTTTTTTAATAAATTTGCCATCGCCCATTCCAATGT
TACGGCCGCAGTATCCGTTCCAGCTAGCATCATACTCTATATACATTAAATTCACACACA
AGTTGTTAGTTCATCTACACATGTACACATATAATATCTTTATAATATAAATGTAAAAAC
ATAAACCATCTTTCTTCTTTCAGTTTTATGTTTGCATTTAGTGAGTCCAAGGAAAAAAAA
TTATATTTAAGGTTTATATGTATGAGAATACTTACGAGCATGAGCCCTTTGATGATGACG
TCACTGTAATACTTGGGTTGGTCTAGTTGTAAAGACAACAAGTGACTAACCATTGTGTTG
CTCTCTCCATTTATTCGACATTCATCGAGCAGTCGTTGTAAGAAAGCGTCCATGGCTTCG
CCTAGTGCTTTCACTTTTTTCTCGTAGCCGTGTCCGAACACTTTAAGAATCGGTAGATAA
TCTCCTGGATGGCTCGCACCACTGTTGTCATTGATGTCCGTCACTAGTTTCTTGAATAGA
TTCGCTTCTTCCTTGTTATGA

Last Updated on 06.12.2018 16:11