SimpleSearch - Line and FST details


Line specific information

 
Line ID 030D12
Vector Used pAC106
Line Availability available as T3 set from NASC (N402832)
Segregation Analysis 50:41:40
Confirmed for Hit At5g57220
Parent of DUPLO pair none
Parent of pair(s) 6180, 6225, 6251, 7760, 7777, 61379, 93723, 93724, 93725, 93726, 93728, 93729, 93730, 93732

Gene hit At1g56310

 
Sequence (A. th genome BLAST matches underlined) 
>62-011521-rosso-D12-35St
TTGACGTAAGGGATGACGCACATCNCACTATCCTTCGCAAGACCCTTCCTCTATATAAGG
AAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTT
CGGTTTGATGTAGAGTATGCACCATAACCTTCTGCATCATAAGTTAAAGATCCACCAACA
GTAGCAAGGAAGAACTGAGCAGCTATCTGACCCGTCGTACAAAGATGTTCTTCACTATGG
GATCTATACTTTCGGGTTGATAATCGAGTATAGAAGACCTCACTAACACTGCAGTGACCC
ACTCCCGCAACCGAAACGCGACACCTATATACTATGACAATTACACTACCTCATAACGTT
ACCACTCCTACACGCTCACCACTCCTCCACCATAACGTATTTCGCCTCCTCCTCACTAAT
CTAAAATCCCCATATTGCAGGAACGAGCGCACAACACCCACATTAGCCTCTTTCACACCC
ACTCCGCCCTCCCACGTAAACTATTCTAACGACAATACTGCGTGCCTGACATACGCCGAC
CAACATCACTACCACTCACGGCGAGTGGTTTCCAAACTATCCATACTCACACATTCATCT
CCACTACCTGTTCATAACCTACTTCTCTATCCCTCAAAGTCCCAAAATTTATAGATACAG
AGCATAACTAAATAACAGAAATACTAGTGGGTTTGACTTGTACTTGATCCTCATGTAAAA
GACGTGGCCAGCACCCACGCCTGTCTGTTTATTAAGTGCACAGGAGAAATATCTTACACG
ACGTAATACTCATTTTGTTCGTCCTTTTCCACACAGATATAAAAAAACACCACAACGCAT
CAGTCATCTGTCCCCAACTAAACCACACACCTCTCAATGATTCAGAAAAACCCAGAAAA
GenBank Accession AL762934 [GenBank]
Graphic View Graphic view of gene At1g56310
Predicted Position of Insertion Chr1:21083391 - go to primer design
BLAST e Value 4e-29
Hit Clone Code (BAC ID) F14G9
Hit Gene Code At1g56310 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Polynucleotidyl transferase, ribonuclease H-like superfamily protein
Insertion Classification CDSi
Confirmation Status unknown

Gene hit At5g57220

 
Sequence (A. th genome BLAST matches underlined) 
>26-011522-rosso-D12-oriL
NTGATCCATGTAGATTTCCCGGACATGATGCCATGTACATTTGTATATTGGACGCAACCG
AAAGAGTCTCGGAACCTATGGTGTGGATATAAGGTAGATTCTCAACGTCAGGTTCATGGA
CCAATCTCTCTTCTCCAATGCTTACTCGTCTATCTCAGCTTTTGCCTTTCTACAACACTT
AAGGTCTGTGAAATAAATTGGCCATCGCCCATCTCAATGTTACGGCCGCACCATCCGTTT
CAGCAATGGGATCTATACTTCCGGGTATGAAACCAAGCACCGGTCAGGCACGCATCCTGA
TCGCGAAGAATTCGAACGCAGGTACAATCGAGCCGACGTTCACGGTACCGGGCCCCCCCT
GGAGGTCGATCCAACCCCTCCGCTGTTATAGTGCAGCCGGCCTCTGACGTTCAGTGCAGC
CGTCTTTTGAAAACGACATGTCGCACAAGTCCTAAGTTACGCGACAGGGCTGCCGCCCTG
CCCTTTTTCCTTGGCGTTTCCTTGGTCGCGTGTTTTAGTCGCATAAAGGTAAAATACTTG
GCCACTTGGGGATCTATACTTTCCGGGTTGAGAAT
GenBank Accession FR800804 [GenBank]
Graphic View Graphic view of gene At5g57220
Predicted Position of Insertion Chr5:23189279 - go to primer design
BLAST e Value 6e-40
Hit Clone Code (BAC ID) MJB24
Hit Gene Code At5g57220 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation cytochrome P450, family 81, subfamily F, polypeptide 2
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details
Other FSTs Supporting this Hit CR395567 [GenBank]


Last Updated on 10.06.2021 13:37