Sequences from confirmation amplicon


Confirmation sequences for line 030F02 and hit At1g80230 are shown.
Line ID 030F02
Locus At1g80230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30170103
Primers used for PCR 02eb - 3144, show primer details
   
Sequence Name 27-K117403-0022-030-F02C-02eb
GenBank Accession LN498574 [GenBank]
Sequencing primer gene specific primer, 02eb: 5'-GAGCAATTGATTTGTTTCTGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117403-0022-030-F02C-02eb
GGGCCTTTTGGAACAAGGTTTGTGTCATTGAAAAGGATAGACTATATTGTATATGATAAG
CAAACCTCTCTCGACTCTTGTCTGAGTGTTATGTTTTCTGATCTATAGGAAGCTCCTGCT
GTAGTCAAATCCTACTACGACATGAGAATAGTTGGTTGCCCTGGAGGTGAAGGAGGTGGG
TCTTTTTTATTTTGCTTTCATTGTGTGCATCATGATTTGGTTTATTTTTAGTAGACCAAA
GGAGATATCACCTCAATCCACAGAGTGTGATACAATCCTGCATCATATCACTGATCACGG
TCGTCCAACCTGTGAAAGATATAGCTTGAGTGATTCTCATCAATACAACAGGAAGTTGCA
AACGTGTTCCATGAC
   
Sequence Name 03-K117402-0022-030-F02C-3144
GenBank Accession LN487660 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117402-0022-030-F02C-3144
TCAGTGCATACACATGCTAAGACCCGCCTCCTTCACCTCCAGGGCAACCAACTATTCTCA
TGTCGTAGTAGGATTTGACTACAGCAGGAGCTTCCTATAGATCAGAAAACATAACACTCA
GACAAGAGTCGAGAGAGGTTTGCTTATCATATACAATATAGTCTATCCTTTTCAATTACA
CAAACCTTTGTTCCAAAAGGCCCTTCAGGAAAGTCTATATCGTCCAGCTTCCTCCCCTGT
CAAAGGTAAACTAATAGCAATCAGAAACAAATCAATTGCTCAGCGGGGTAGAGGTGATCC
CGCCTTGGTCGAAAGTCAATGTTCTCACCCTGAAGTATGGTTGTGCCCTCCAAGCACGGA
ACCGCAATGCTGACCGTACGGGTCGGATGGTGCACCAATCTGTAGCCATCCAAATCCCCG
AGCCTGTCATACTC

Last Updated on 06.12.2018 16:11