Sequences from confirmation amplicon


Confirmation sequences for line 030H08 and hit At1g71530 are shown.
Line ID 030H08
Locus At1g71530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26940547
Primers used for PCR 08ne - 8474, show primer details
   
Sequence Name 31-K114376-0022-030-H08C-08ne
GenBank Accession LN500262 [GenBank]
Sequencing primer gene specific primer, 08ne: 5'-AGAGTTCATTACTTCTGTGCGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114376-0022-030-H08C-08ne
CAGCCGGCGCTCCACAGGTAATGGCTGGTCCATACTCAGTGGCACCGAGCAACAGCTCAG
GTGCTCTGTACCATAAGGTCACAACACGACTGGTCAGCTGCAGATCTCCATCACCTCGGT
AAAAATTCGCCAATCCGAAATCACCAATCTTCAAGACACCTTCATTATTGATTAAGAGAT
TTGAGCCTTTAATATCACGGTGAAGAATTCCTCGTCTGTGGCAATGTTCAAGCCCACGGA
ACAATTGCTGCATATAGCACTTGATCTGAAAACGAAGTAGGACTCTTTATAAAACTTGAA
TTCTCCAATCTTTCAAGTGCATAGGAAAACAGAATATGTAATACCTGAGGTTCAGAGAAT
TTGATTCCAGGAGTTGCAGCTAGACCTGCGAGATCGTGTTCCATGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCACGGTT
   
Sequence Name 17-K114375-0022-030-H08C-8409
GenBank Accession LN493947 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114375-0022-030-H08C-8409
ATTTACTGGAACCGATCTCGCAGGTCTAGCTGCAACTCCTGGAATCAAATTCTCTGAACC
TCAGGTATTACATATTCTGTTTTCCTATGCACTTGAAAGATTGGAGAATTCAAGTTTTAT
AAAGAGTCCTACTTCGTTTTCAGATCAAGTGCTATATGCAGCAATTGTTCCGTGGGCTTG
AACATTGCCACAGACGAGGAATTCTTCACCGTGATATTAAAGGCTCAAATCTCTTAATCA
ATAATGAAGGTGTCTTGAAGATTGGTGATTTCGGATTGGCGAATTTTTACCGAGGTGATG
GAGATCTGCAGCTGACCAGTCGTGTTGTGACCTTATGGTACAGAGCACCTGAGCTGTTGC
TCGGTGCCACTGAGTATGGACCAGCCATTGACCTGTGGAGCGCCGGCTGCATTCTCACTG
AGCTCTTCGCCGGAAAACCTATNATGCCAGGACGCANAGAAGTAATGAACTC

Last Updated on 06.12.2018 16:11