Sequences from confirmation amplicon


Confirmation sequences for line 031E02 and hit At2g44070 are shown.
Line ID 031E02
Locus At2g44070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18232260
Primers used for PCR 39gz - 8474, show primer details
   
Sequence Name 61-K112176-0022-031-E02C-39gz
GenBank Accession LN510560 [GenBank]
Sequencing primer gene specific primer, 39gz: 5'-GAACTTATAGGCTTCGCAACAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K112176-0022-031-E02C-39gz
ACACAAGCAGTGCCAACCCTTGAGTAAACTGTTCCGTTAGAAAAAACTGATGAGGCGCCC
AAAAACACTTTGGTGACTTGGTGCATGATATATGATATGGCAGTTATATGAGTGTATGTA
CAGTTAATGCCTCGTTTGATCAATCTACGAAGCAGTAGCTTCCCTTGAAGCTTTGGTCGA
GAATCCACCACAAAGACACGAAACTTTTTCCCGAGTTCATGTGCATGCACTAATATCATC
TCAATGACAGTGGAAGATCCGTAGGTCAGAAGGACATCACCGTCTCTTATTTTAGTGACA
GCATGTTCGACTATCACTGTATCTGCACCTATGATTTTCTCGTTAATGAAACGTTCAATA
TCTGAGTGAAGAGAAGCTTTTGCTTCAGATTCAGAAAGTGTTATAGGCAGCTTTGCAATC
CTGTTTTTCACAAATCGGATTGCGTTTCCCATGCTAATCGAAAGCGGACGGCATTCTATG
AGGAAAGAGACGTAGCTACTTATTTGAGCAGTCATGTCTCTATTAAGGGTATTCTCAGGC
GGCGTCGAGTAATCTTTAACAACCTCTTGGTATATACATTTATCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 45-K112175-0022-031-E02C-8409
GenBank Accession LN505576 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K112175-0022-031-E02C-8409
ATGTATATACCAAGAGGTTGTTAAAGATTACTCGACGCCGCCTGAGAATACCCTTAATAG
AGACATGACTGCTCAAATAAGTAGCTACGTCTCTTTCCTCATAGAATGCCGTCCGCTTTC
GATTAGCATGGGAAACGCAATCCGATTTGTGAAAAACAGGATTGCAAAGCTGCCTATAAC
ACTTTCTGAATCTGAAGCAAAAGCTTCTCTTCACTCAGATATTGAACGTTTCATTAACGA
GAAAATCATAGGTGCAGATACAGTGATAGTCGAACATGCTGTCACTAAAATAAGAGACGG
TGATGTCCTTCTGACCTACGGATCTTCCACTGTCATTGAGATGATATTAGTGCATGCACA
TGAACTCGGGAAAAAGTTTCGTGTCTTTGTGGTGGATTCTCGACCAAAGCTTCAAGGGAA
GCTACTGCTTCGTAGATTGATCAAACGAGGCATTAACTGTACATACACTCATATAACTGC
CATATCATATATCATGCACCAAGTCACCAAAGTGTTTTTGGGCGCCTCATCAGTTTTTTC
TAACGGAACAGTTTACTCAAGGGTTGGCACTGCTTGTGTTGCAATGGTGGCTAATGCTTT
CCGTGTTCCTGTGCTTGTTTGTTGCGA
Primers used for PCR 47lf - 8474, show primer details
   
Sequence Name 75-K113840-0022-031-E02C-47lf
GenBank Accession LN513280 [GenBank]
Sequencing primer gene specific primer, 47lf: 5'-GAGCATATTGAATAATCCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113840-0022-031-E02C-47lf
GCTTGCACAACAAGCACAGGAACACGGAAAGCATTAGCCACCATTGCAACACAAGCAGTG
CCAACCCTTGAGTAAACTGTTCCGTTAGAAAAAACTGATGAGGCGCCCAAAAACACTTTG
GTGACTTGGTGCATGATATATGATATGGCAGTTATATGAGTGTATGTACAGTTAATGCCT
CGTTTGATCAATCTACGAAGCAGTAGCTTCCCTTGAAGCTTTGGTCGAGAATCCACCACA
AAGACACGAAACTTTTTCCCGAGTTCATGTGCATGCACTAATATCATCTCAATGACAGTG
GAAGATCCGTAGGTCAGAAGGACATCACCGTCTCTTATTTTAGTGACAGCATGTTCGACT
ATCACTGTATCTGCACCTATGATTTTCTCGTTAATGAAACGTTCAATATCTGAGTGAAGA
GAAGCTTTTGCTTCAGATTCAGAAAGTGTTATAGGCAGCTTTGCAATCCTGTTTTTCACA
AATCGGATTGCGTTTCCCATGCTAATCGAAAGCGGACGGCATTCTATGAGGAAAGAGACG
TAGCTACTTATTTGAGCAGTCATGTCTCTATTAAGGGTATTCTCAGGCGGCGTCGAGTAA
TCTTTAACAACCTCTTGGTATATACATTTATCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 67-K113841-0022-031-E02C-8409
GenBank Accession LN511859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113841-0022-031-E02C-8409
TCGGATGAGCATTTCATTGATAAATGTATATACCAAGAGGTTGTTAAAGATTACTCGACG
CCGCCTGAGAATACCCTTAATAGAGACATGACTGCTCAAATAAGTAGCTACGTCTCTTTC
CTCATAGAATGCCGTCCGCTTTCGATTAGCATGGGAAACGCAATCCGATTTGTGAAAAAC
AGGATTGCAAAGCTGCCTATAACACTTTCTGAATCTGAAGCAAAAGCTTCTCTTCACTCA
GATATTGAACGTTTCATTAACGAGAAAATCATAGGTGCAGATACAGTGATAGTCGAACAT
GCTGTCACTAAAATAAGAGACGGTGATGTCCTTCTGACCTACGGATCTTCCACTGTCATT
GAGATGATATTAGTGCATGCACATGAACTCGGGAAAAAGTTTCGTGTCTTTGTGGTGGAT
TCTCGACCAAAGCTTCAAGGGAAGCTACTGCTTCGTAGATTGATCAAACGAGGCATTAAC
TGTACATACACTCATATAACTGCCATATCATATATCATGCACCAAGTCACCAAAGTGTTT
TTGGGCGCCTCATCAGTTTTTTCTAACGGAACAGTTTACTCAAGGGTTGGCACTGCTTGT
GTTGCAATGGTGGCTAATGCTTTCCGTGTTCCTGTGCTTGTTTGTTGCGAAGCCTATAAG
TTCCATGAGAAGGTGCAGTTGGATTATCNAATATGCT

Last Updated on 06.12.2018 16:11