SimpleSearch - Line and FST details
Line specific information
| Line ID | 031E02 |
| Vector Used | pAC106 |
| Line Availability | available as T3 set from NASC (N402930) |
| Segregation Analysis | 50:33:26 |
| Confirmed for Hit | At2g44070 At3g21340 At2g46290 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 1187, 6698, 6699, 6700, 6701, 6702, 6704, 6707, 6710, 6713, 6715, 6720, 6721, 6722, 6727, 6734, 6741, 6745, 6752, 8953, 72878, 72879, 72880, 72881, 72882, 72884, 72885, 72887, 72889, 72890, 72891, 72892, 72893, 72895, 72896, 72897 |
| Note |
Gene hit At2g44070
| Sequence (A. th genome BLAST matches underlined) | >13-K113047-0022-031-E02-8409 CCAAGAGGTTGTTAAAGACTACTCGACGCCGCCTGAGAATGCTCTTACTAGAGACATGAA TGGACGAATAATTAGCTACGTTCTCATTCCGCATAGAAGGCCGGCCAGAATTCGATTAGC ATGGGAAACGCTTTCAGATGTTGTGAAAAAGAGGATTGCAATGCTGCCTATAACACTTTC TGAATCTGAAGCAAAAGCTTCTCTTCACTCATATATTGGACGTTTCGTTAACGAGAAAAT CATAGGTGCAGATACAGTGATAGTCGAACATGCTGTCACTAAAATAAGAGACGGTGATGT CCTTCTGACCTACGGATCTTCCACTGTCATTGAGATGATATTAGTGCATGCACATGAACT CAGGAAAAAGTTTCGTGTCTTTGTGGTGGATTCTCGACCAAAGCTTCAAGGGAAGCTACT GCTTCGTATATTGATCAAACGAGGCATTAACTGTACATACACTCATATAACTGCCATATC ATATATCATGCACCAAGTCACCAAAGTGTTTTTGGGCGCCTCATCAATTTTTTCTAACGG AACAGTTTACTCTTGGGTTGGCACTGCTTGTGTTGCAATGGTGGCTAATGCTTTCCGTGT TCCTGTGCTTGTTTGTTGCGAAGCCTATCAGTTCCATAAGAAGGTGCAGTTGGATTATTC AATATGCTCCGATGAGCTCGGTGATCCAAATGCTATTTCAAAAGTTCATGGAAGAGAAGA CATAAACTACTTAAATGGCTTGACCAACAATGCAAAGCTGAAGTTTCTGAACTTGATGTA AGCTAACTGCCCGGGCCAGCTAAAAGNNNGGGNCGGCCNGGCGTTTCNTTGGTGGCTAGG GCTATCCGTGTTCCTGTGCCTGGTTGTTTCNAAGCC |
| GenBank Accession | FR800878 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:18232260 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | F6E13 |
| Hit Gene Code | At2g44070 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | NagB/RpiA/CoA transferase-like superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | FR800878 [GenBank] |
Gene hit At5g44565
| Sequence (A. th genome BLAST matches underlined) | >13-K014862-022-031-E02-8409 TTTTGATCCATGTAGAATTTCCCGGACATGAANGCCTTTACAATTGAATATATCCTTGAA TATAAATACTTACCTGACATTTATAGGTGCCTTTATGTGTTGATTCATATGACAGATATC GAGGGGGATCTATTCTAACATGCGAACCTAAGACATTCTCCGTTTATATGAAGACGACTG TATATCAATAATGTCATATCTGTCTACCTAAATAAAGAATAAATATCTTTGTTACGTACA CAGAAAGAAAGAATAAATATCTTTAAGACTAAAATGAAGGGAGGAGAATGATGGCGAATT ATAAGCCCAATACAGCAGTAGAAACACCACCATCAATAAGTATACCAGGTGGTGGTGGTC AGAATACGGTACTCACAATTCATTACTTTAAAATAAATCAATAATTGATCACCTCCTTAA TTTAATAATTATGCACTTTGCTTCTAATATATATTAAATTTGCCTATGGGATCCT |
| GenBank Accession | AL762992 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:17952395 - go to primer design |
| BLAST e Value | 5e-142 |
| Hit Clone Code (BAC ID) | MFC16 |
| Hit Gene Code | At5g44565 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | transmembrane protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 031E03 |
Gene hit At3g21340
| Sequence (A. th genome BLAST matches underlined) | >13-K113048-0022-031-E02-8409 GACATATAAATACTTACCTGACATTTATAGTTGCCATTTATATGTTGATTCAAATGTAGG AAAGCGAGGTGGATCTATTCTAAATTGGGAAAGTAACCTGCCTTGCCGCTAACGCTGCGG ACATCTACATTTTTGAATTGAAAAAAAATTGGTAATTACTCTTTCTTTTTCTCCATATTG ACCATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGA ATATATCCTGACATATAAATACTTACCTGACATTTATAGTTGCCATTTATATGTTGATTC AGGTGTAGGAAAGCAAGGTGGATCTATTCTAAATTGGGAAACTAACCTGCCC |
| GenBank Accession | FR800879 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:7512787 - go to primer design |
| BLAST e Value | 1e-23 |
| Hit Clone Code (BAC ID) | MHC9 |
| Hit Gene Code | At3g21340 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Leucine-rich repeat protein kinase family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At2g46290
| Sequence (A. th genome BLAST matches underlined) | >82-K119172-0022-031-E02C-3144 AGTGGATGGAATGGATTCAGTTTAGGATTTAAGTCGTTGTCTTTGTTGTTTTAGTCTTTC ATAGCTTAACGATTAGTGTTTTGTTTGTGATTTCTTAGGAGACTCGTCTAGATTGATCAC TGGTAGTGCTGATCAGACTGCAAAGCTGTGGGATGTGAAATCTGGCAAAGAATTGTTCAC TTTCAAGTTTGGTGCCCCTGCAAGGTCTGTGGATTTCTCTGTTGGTGATCATCTTGCAGT GATTACCACTGATCACTTCGTGGGAACTTCCTCTGCTATTCATGTCAAACGCATTGCAGA AGATCCCGAAGACCGTAAATTGTCAATTCT |
| GenBank Accession | LM644487 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr2:19007392 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | T3F17 |
| Hit Gene Code | At2g46290 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Transducin/WD40 repeat-like superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
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Last Updated on 10.06.2021 13:37 |
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