Sequences from confirmation amplicon


Confirmation sequences for line 031E02 and hit At3g21340 are shown.
Line ID 031E02
Locus At3g21340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7512767
Primers used for PCR KE40 - 8474, show primer details
   
Sequence Name 22-K100995-0022-031-E02C-8409
GenBank Accession LN496038 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K100995-0022-031-E02C-8409
TTTACATTGAATATATCCTGACATATAAATACTTACCTGACATTTATAGTTGCCATTTAT
ATGTTGATTCAAATGTAGGAAAGCGAGGTGGATCTATTCTAAATTGGGAAACTAGACTAA
AAATAGTTGTCGAGTCTGCCCAAGGTTTGATATGAGTTTTAATATAATTTTTGCTTGTAT
CCTCTATTCATTTGCTTAACCCTTGATGAAAATGAAATTTGTTGCATAGGTTTGGAATAC
TTGCATAATGGATGCAAACCACCAATGGTTCATAGGGATGTTAAAACCACAAATATATTG
TTGAATGAACACCTCCATGCTAAGCTAGCTGATTTTGGGCTTTCGAGATCTTTTCCAATT
GAAGGAGAAACTCATGTGTCAACAGTTGTTGCTGGAACTCCTGGATACCTTGATCCAGAG
TAAGTTTCATAATTGTTGGGCAATGATTTCCNTTTTAA
   
Sequence Name 22-K100999-0022-031-E02C-KE40
GenBank Accession LN496039 [GenBank]
Sequencing primer gene specific primer, KE40: 5'-TTTAAAAGAAAATCATTGCCCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K100999-0022-031-E02C-KE40
GGAGTTCCAGCAACAACTGTTGACACATGAGTTTCTCCTTCAATTGGAAAAGATCTCGAA
AGCCCAAAATCAGCTAGCTTAGCATGGAGGTGTTCATTCAACAATATATTTGTGGTTTTA
ACATCCCTATGAACCATTGGTGGTTTGCATCCATTATGCAAGTATTCCAAACCTATGCAA
CAAATTTCATTTTCATCAAGGGTTAAGCAAATGAATAGAGGATACAAGCAAAAATTATAT
TAAAACTCATATCAAACCTTGGGCAGACTCGACAACTATTTTTAGTCTAGTTTCCCAATT
TAGAATAGATCCACCTCGCTTTCCTACATTTGAATCAACATATAAATGGCAACTATAAAT
GTCAGGTAAGTATTTATATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR 05lp - 8474, show primer details
   
Sequence Name 02-K114006-0022-031-E02C-8409
GenBank Accession LN487119 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114006-0022-031-E02C-8409
ATTTCATTGAATAATCCTGACATATAAATACTTACCTGACATTTATAGTTGCCATTTATA
TGTTGATTCAAATGTAGGAAAGCGAGGTGGATCTATTCTAAATTGGGAAACTAGACTAAA
AATAGTTGTCGAGTCTGCCCAAGGTTTGATATGAGTTTTAATATAATTTTTGCTTGTATC
CTCTATTCATTTGCTTAACCCTTGATGAAAATGAAATTTGTTGCATAGGTTTGGAATACT
TGCATAATGGATGCAAACCACCAATGGTTCATAGGGATGTTAAAACCACAAATATATTGT
TGAATGAACACCTCCATGCTAAGCTAGCTGATTTTGGGCTTTCGAGATCTTTTCCAATTG
AAGGAGAAACTCATGTGTCAACAGTTGTTGCTGGAACTCCTGGATACCTTGATCCAGAGT
AAGTTTCATAATTGTTGGGCAATGATTTTCTTTTAAATTTTATTTTAAGGGTAATTGCTA
GTTTTCATCTATCATGAGTATCATCACTTGATAGGATATCATATATATTTTTTATTTTTA
ATAGGATAAATATTGATTTTGCAGATATTACCGA
   
Sequence Name 01-K114005-0022-031-E02C-05lp
GenBank Accession LN486658 [GenBank]
Sequencing primer gene specific primer, 05lp: 5'-TTCGGTAATATCTGCAAAATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K114005-0022-031-E02C-05lp
TATGATATCCTATCAGNTGATGATACTCATGATAGATGAAAACTAGCAATTACCCTTAAA
ATAAAATTTAAAAGAAAATCATTGCCCAACAATTATGAAACTTACTCTGGATCAAGGTAT
CCAGGAGTTCCAGCAACAACTGTTGACACATGAGTTTCTCCTTCAATTGGAAAAGATCTC
GAAAGCCCAAAATCAGCTAGCTTAGCATGGAGGTGTTCATTCAACAATATATTTGTGGTT
TTAACATCCCTATGAACCATTGGTGGTTTGCATCCATTATGCAAGTATTCCAAACCTATG
CAACAAATTTCATTTTCATCAAGGGTTAAGCAAATGAATAGAGGATACAAGCAAAAATTA
TATTAAAACTCATATCAAACCTTGGGCAGACTCGACAACTATTTTTAGTCTAGTTTCCCA
ATTTAGAATAGATCCACCTCGCTTTCCTACATTTGAATCAACATATAAATGGCAACTATA
AATGTCAGGTAAGTATTTATATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA

Last Updated on 06.12.2018 16:11