Sequences from confirmation amplicon


Confirmation sequences for line 031E09 and hit At4g34200 are shown.
Line ID 031E09
Locus At4g34200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16375314
Primers used for PCR 11jx - 8474, show primer details
   
Sequence Name 63-K113261-0022-031-E09C-11jx
GenBank Accession LN511028 [GenBank]
Sequencing primer gene specific primer, 11jx: 5'-TCGTTACATTTGCTGATTCTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K113261-0022-031-E09C-11jx
CTGACTTGTGGATCTTGGTTTTAATAATACCATTGTAATGCAGGAGAGTGGAAGAGGAAC
AAGTATGTAGGTGTTTCACTTGTGGGAAAGACCCTTGCAGTGTTGGGATTCGGGAAAGTT
GGGACGGAAGTTGCTCGTCGTGCTAAGGGTCTTGGTATGCGTGTCATTGCTCATGATCCC
TATGCCCCAGCAGACCGTGCACACGCCATTGGTGTTGACTTAGTGAGCTTTGATGAAGCC
CTTGCCACCGCGGATTTCATTTCTCTTCATATGCCTCTTACGCCAACAACATCGAAGATA
CTAAACGACGAAACCTTTGCCAAAATGAAGAAGGGAGTTCGAATTGTTAATGTTGCTCGT
GGAGGTGTCATAGACGAAGATGCGTTAGTGAGAGCTCTGGATGCTGGTATTGTTGCGCAG
GCTGCACTTGATGTTTTCACCAAAGAGCCTCCGGCTAAAGATAGATCAGCAATGGCTTCA
ATTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 44-K113260-0022-031-E09C-8409
GenBank Accession LN505247 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113260-0022-031-E09C-8409
TCGGAATGAGCCATTTACATTGAATATATCCTGAAAATTGAAGCCATTGCTGATCTATCT
TTAGCCGGAGGCTCTTTGGTGAAAACATCAAGTGCAGCCTGCGCAACAATACCAGCATCC
AGAGCTCTCACTAACGCATCTTCGTCTATGACACCTCCACGAGCAACATTAACAATTCGA
ACTCCCTTCTTCATTTTGGCAAAGGTTTCGTCGTTTAGTATCTTCGATGTTGTTGGCGTA
AGAGGCATATGAAGAGAAATGAAATCCGCGGTGGCAAGGGCTTCATCAAAGCTCACTAAG
TCAACACCAATGGCGTGTGCACGGTCTGCTGGGGCATAGGGATCATGAGCAATGACACGC
ATACCAAGACCCTTAGCACGACGAGCAACTTCCGTCCCAACTTTCCCGAATCCCAACACT
GCAAGGGTCTTTCCCACAAGTGAAACACCTACATACTTGTTCCTCTTCCACTCTCCTGCA
TTACAATGGTATTATTAAAACCAAGATCCACAAGTCAGCTAGATAACAGACAAGAATGAT
CAAAACTCAATTTCAAAAGAATCAGCAAATGTAACGA

Last Updated on 06.12.2018 16:11