Sequences from confirmation amplicon


Confirmation sequences for line 031F08 and hit At1g66910 are shown.
Line ID 031F08
Locus At1g66910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24963430
Primers used for PCR 12jx - 8474, show primer details
   
Sequence Name 66-K113379-0022-031-F08C-12jx
GenBank Accession LN511652 [GenBank]
Sequencing primer gene specific primer, 12jx: 5'-GTCTCTTGTACCCTTTCTGGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K113379-0022-031-F08C-12jx
CGCAGGAGTCCCAGAGCTTAGCATTTCTTCTGTCAAATTTAGAATCCTAGGTGCGGATTA
TGACTCTGGTATCATAACACTCGCCAGATCCGATAATATCGACGATCCGTGTCTCCCAAA
TTCCTTCACTACGTCATTCAACGAAACCGTCCTTCCACTTGCTTCTACCACCGACCTGCT
AACCATTTATTACGACTGCAACCGAAACGTTTCATCGTTTGTTTCTACTTTTGTTAAAGA
GCTTGATTGTCCAGATGATGGGACTGATGATAGAAGAAATTATTATCTGACGAGAAATCA
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 28-K113377-0022-031-F08C-8409
GenBank Accession LN498927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K113377-0022-031-F08C-8409
TCGGACTGAGCATTTCATGATTTCTCGTCGATAATAATTTCTTCTATCATCAGTCCCATC
ATCTGGACAATCAAGCTCTTTAACAAAAGTAGAAACAAACGATGAAACGTTTCGGTTGCA
GTCGTAATAAATGGTTAGCAGGTCGGTGGTAGAAGCAAGTGGAAGGACGGTTTCGTTGAA
TGACGTAGTGAAGGAATTTGGGAGACACGGATCGTCGATATTATCGGATCTGGCGAGTGT
TATGATACCAGAGTCATAATCCGCACCTAGGATTCTAAATTTGACAGAAGAAATGCTAAG
CTCTGGGACTCCTGCGTTACAGTCAAGCTCGAAGTCAGGGTGACCGCATTCTTTTCTGCC
AGCAATCCAGAAAGGGTACAAGAGA

Last Updated on 06.12.2018 16:11