Sequences from confirmation amplicon


Confirmation sequences for line 031F08 and hit At3g28350 are shown.
Line ID 031F08
Locus At3g28350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10609865
Primers used for PCR 41eq - 8474, show primer details
   
Sequence Name 49-K113531-0022-031-F08C-41eq
GenBank Accession LN506907 [GenBank]
Sequencing primer gene specific primer, 41eq: 5'-GACACCTCCTTATCCTACAATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113531-0022-031-F08C-41eq
AGAATCTATATATATATATGATGTCTTTTCCTGAGTATCCTGTTTAAGAAGACTACAAAA
ATCTATTTATGAGCCTGAACTAGACACTAGCTGCTTCAACCTGTCTAAGAGAAATCTGGT
AGGTCTATATGTGATTGATGCTTTCTATATTTCTTAACTCTTGAGATTTCGGTTAGGGTT
CATTGGTTTTGGTACAGCAAATAGTTTTTTGTACAGGTAGAGAGATATCTGAACACGGAT
ACATCATGCTGACCAATACCATAAAAACATTAATGCATTTGTTTCCAGTAATTCCATGGC
ATGTCGAAAATGGTATATAGAAATGAGAAAAATAGTTGGTAAGGGTCAACATACTTGATC
TCCCTGAAGAAGTAAGCTCAAAACTTCCAAATTCTCTGCTTGGCATTGTTGACATCGGCA
TTTCTTTTGCAGCTCCTCCCCGAGTGAAGCCAATATCTGTTCCAACTCGGACGTCTGCTT
GTTTTCTGTCAAGGGAACTATTCTTTTGAAAGAATGGCAAGTCAACCTGAAAAGTACAAG
CACTGGCTTGCTTTTCTATCCTAGGAAAAAATTGCCTTGCTGTAGAGTACAGTACATGCC
GCCTCAATAAAGAACAGAACTAGCAGGTGAAAATGTAGATGTCCGCAGCGTTA
   
Sequence Name 24-K113530-0022-031-F08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K113530-0022-031-F08C-8409
ATCCTGCCGCCGCTGCCACTATGCACCCGGTGGAGCTTGCATGTTGGTTACTACGCAGAA
CTGAGCCGGTTAGGCACATAATTTCCATTGTAGGATAAGGAGGTGTC

Last Updated on 06.12.2018 16:11