Sequences from confirmation amplicon


Confirmation sequences for line 031G02 and hit At1g35710 are shown.
Line ID 031G02
Locus At1g35710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13222792
Primers used for PCR RE30 - 8474, show primer details
   
Sequence Name 09-K025445-022-031-G02C-8409
GenBank Accession LN490020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K025445-022-031-G02C-8409
ATTTCCCGGACATGAAACCATTTACAATTAAGTCGTAGCCTCGACAAATTCGTGAGATTT
CCAATTGCTTCTGGAAGTTCACCAAAGAGGTTGTTGGTAGATAGATCCAGCTCAACTAGT
TGTGTCATGTTCCAAATCTCTGTTGGGATAGCACCCGTGATGTTGTTGTTTGACATGATC
AAGGCACCTAGCTTTGGACTCTTCTCCCAGTTGCTAGAAATCTCACCGTGGAATTTGTTG
TGGCTGAAATCAATGAAGTTAAGGTCTGGGTAAATCCCAAAAGCTTCAAAAATATCGCCA
GTGAATTTGTTTCCTAGAAATCTTGCTCTGATCAGGCTCTTGCAATCTCTCAAGCTTTTC
GGGATAGGACCTTCGAGGTGATTGTAATCCAATGAGATATTTTGAAGCTTCCTACCTTTG
CAAACAGTTTCAGGAAAGAAACCAGTGAAGTTGTTGGTGTCAAGTATCAAGGTAGTTAGA
TGTGAAGAGTTTGCTACTCCTGGCGGGATCGCACCAGAGAGGTGGTTAACACG
   
Sequence Name 10-K025445-022-031-G02C-RE30
GenBank Accession LN490493 [GenBank]
Sequencing primer gene specific primer, RE30: 5'-TGGAAAGTTTGTACCTTCGTGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K025445-022-031-G02C-RE30
CACCTCTCTGGTGCGATCCCGCCAGGAGTAGCAAACTCTTCACATCTAACTACCTTGATA
CTTGACACCAACAACTTCACTGGTTTCTTTCCTGAAACTGTTTGCAAAGGTAGGAAGCTT
CAAAATATCTCATTGGATTACAATCACCTCGAAGGTCCTATCCCGAAAAGCTTGAGAGAT
TGCAAGAGCCTGATCAGAGCAAGATTTCTAGGAAACAAATTCACTGGCGATATTTTTGAA
GCTTTTGGGATTTACCCAGACCTTAACTTCATTGATTTCAGCCACAACAAATTCCACGGT
GAGATTTCTAGCAACTGGGAGAAGAGTCCAAAGCTAGGTGCCTTGATCATGTCAAACAAC
AACATCACGGGTGCTATCCCAACAGAGATTTGGAACATGACACAACTAGTTGAGCTGGAT
CTATCTACCAACAACCTCTTTGGTGAACTTCCAGAAGCAATTGGAAATCTCACGAATTTG
TCGAGGCTACGACTTAATTGTAAATGGTTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATNTCGACGTGTCTACA
Primers used for PCR FL58 - 3144, show primer details
   
Sequence Name 91-K028754-022-031-G02C-3144
GenBank Accession LN515078 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K028754-022-031-G02C-3144
CGGTTTAAAACTCTCATCCGAATATATCCCGCAGATTGTCGTTTCCCGTCTATCTGGAAG
AGTTCCTGCAGGTTTAAGTTTTCTTAACCAATCTTGAGTCTCTCGACTTATCCTCAAACA
ATTTCAGCTCAGAGATCCCACAAACCTTTGACTCCTTTTTAAAGCTTCATGACATGAACC
TGAGCAGAAACAAATTCGACGGAAGCATTCCCCGACTATCAAAGCTGACTCAGTTAACGC
AACTTGATCTCAGCCACAACCAGCTCGACGGAGAAATCCCATCACAACTAAGCTCCCTGC
AAAGCCTCGACAAGCTCGACCTCTCACACAACAATCTCTCAGGTCTCATTCCAACAACTT
TCGAGGGCATGATAGCGCTGACGAACGTCGATATATCAAACAATAAACTCGAAGGTCCGC
TTCCAGATACTCCAACGTTTCGAAAAGCAACAGCAGATGCATTGGAGGAAAACATAGGCT
TATGCAGTAATATTCCTAAACAAAGATTGAAACCATGCCGTGAGTTGAAGAAACCGAAGA
AAAATGGCAACCTTGTTGTG
   
Sequence Name 91-K028754-022-031-G02C-FL58
GenBank Accession LN515079 [GenBank]
Sequencing primer gene specific primer, FL58: 5'-ATTGGCACTAATATCCACACAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K028754-022-031-G02C-FL58
TTAGGAATATTACTTGCATAAGTCCTATTGTTTTCCTCCAATGCATCTGCTGTTGCTTTT
CGAAACGTTGGAGTATCTGGAAGCGGACCTTCGAGTTTATTGTTTGATATATCGACGTTC
GTCAGCGCTATCATGCCCTCGAAAGTTGTTGGAATGAGACCTGAGAGATTGTTGTGTGAG
AGGTCGAGCTTGTCGAGGCTTTGCAGGGAGCTTAGTTGTGATGGGATTTCTCCGTCGAGC
TGGTTGTGGCTGAGATCAAGTTGCGTTAACTGAGTCAGCTTTGATAGTCGGGGAATGCTT
CCGTCGAATTTGTTTCTGCTCAGGTTCATGTCATGAAGCTTTAAAAAGGAGTCAAAGGTT
TGTGGGATCTCTGAGCTGAAATTGTTTGAGGATAAGTCAAGAGACTCAAGATTGGTTAAG
AAACTTAAACCTGCAGGAACTCTTCCAGATAGACGGGAAACGACAATCTGCAGGATATAT
TCAGATGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCC
TCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATT
GTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGG
TTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGG

Last Updated on 06.12.2018 16:11