Sequences from confirmation amplicon


Confirmation sequences for line 031G02 and hit At3g53570 are shown.
Line ID 031G02
Locus At3g53570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19864342
Primers used for PCR DC83 - 8474, show primer details
   
Sequence Name 05-K032598-022-031-G02C-8409
GenBank Accession LN488240 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K032598-022-031-G02C-8409
GTGTGCTATGAGCCCGGATTCAGCGAATGGGTTATTTCCCAACTTGGATTACCCCCTCCT
GTCTCAAAGGGGCCCTAAGNTTGCGAGTGGTTTAGTTCCCAATTTTGTTTACCCAAACAT
GTTCAAAAA
   
Sequence Name 05-K032598-022-031-G02C-DC83
GenBank Accession LN488241 [GenBank]
Sequencing primer gene specific primer, DC83: 5'-AAGGCCATTATAGAACATGTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K032598-022-031-G02C-DC83
CACTCGCAACTCCGGACCAAGTACACGGAGGTTGAAATACCTGAAAGAAAAAAACACATC
AGCTGAAACTTAGCTCAAGCACACAAATAAAAATCTTAATTTCTTACTAGTTAACACAAT
TCGCAGTATCTTATGAAAACAACAAAAGAACAGTCCATTGATACCAATACAATATGTGGA
CAATTCGTTACCGTGGGTGGAGGAGGAGGAGGAAGAAGAGGCGGAGCAGCATCCCAAGTC
AATCGGGGACGTTTCCTGGGACGCTTATCAACGATCCGATGAGGGAATTCAACATTCCTC
TGAGTTTCTAAGATCATCGCGATCGAGCTAGCTTTATCACGATACACACTGCTTTGCATA
AAGGATTAGAAAAATGCAAATCCAGAGCTGAAATTCAGAAACCCAGATTATGAACAGCGA
GAGAATTGCGATTGAATTTCGAATCTAATTAGAGGGATAATAAGATTCCGAAATTCGAAA
CCCTAGTTATCTTCAATTTGGGGGGGATCTATCTCCAGAAGATAACTAAAAAAGGTGGGA
AAAGTAACAGAAAAATATATTAAAAAAAGTGGAAGTCGCTGTAGCAATGAGTATGATGGT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTAC

Last Updated on 06.12.2018 16:11