Sequences from confirmation amplicon


Confirmation sequences for line 031G10 and hit At5g42360 are shown.
Line ID 031G10
Locus At5g42360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16937613
Primers used for PCR DC73 - 8474, show primer details
   
Sequence Name 08-K117496-0022-031-G10C-8409
GenBank Accession LN489910 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117496-0022-031-G10C-8409
TTGAATAATAGAAGATTACCGCAAGCACGTTTATCTTCCCGATGATATTCTGGAAATGTG
TTTGATGAGGCTTCCTTTGACCAGCTTATTGAATGCACACNCTTGTTTGCAAGAAATGGC
AATCCATGGCCAACACTCAGCGGTTTCTCCAGATGAGACGAGAAGGCTCCTTTCAAACAC
CGTGGTTGTTTCTGTTTGCTGCTCTTAAAGATGGATGCTCCTCTGGTGATATTCACGGGT
ATGATGTTTCTCAGGACAAATGGCATAGGATTGAAACTGATCTACTTAAAGGCCGGTTTA
TGTACTCCGTGACAAGCATC
Primers used for PCR DC74 - 8474, show primer details
   
Sequence Name 36-K118262-0022-031-G10C-DC74
GenBank Accession LN502395 [GenBank]
Sequencing primer gene specific primer, DC74: 5'-AAGAAGCTTTGATTGATTTGCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118262-0022-031-G10C-DC74
AACCGTTGGGAGAAGAATCTATACGGCAGGATCTGGAGGTTTTAACCGTTTCGAGGAGGC
TTGTGAAAAGTGTTAGCCAGAAGTTGAAGAAAAAAATTCATAAAACTGAGGTGGTGGAAG
ATGAAGAGGTTGCAAGGGGAGCGGTGAATTGTTTAAGCATCAGTGTTGGTTGCAGAGTTG
CTGATACGGGTGAGGATTTTGAGGAGGATTTAAGTAATAAGAGATGGTCTAGTGCTAGTG
AGGATGGTAAGGGATTGATGACCATTTGTGGAACTGAAGAAATTAGGTTAGATTGTTTTT
CTTATGGGGTTAGAGAGAGGTTCTGGAAGAAGAATAATAGAAGATACCTTCAATTAAACG
ACAATCTGTTAGCCATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTA
   
Sequence Name 16-K118261-0022-031-G10C-8409
GenBank Accession LN493625 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118261-0022-031-G10C-8409
TTACATTGATATACATGGCTAACAGATTGTCGTTTAATTGAAGGTATCTTCTATTATTCT
TCTTCCAGAACCTCTCTCTAACCCCATAAGAAAAACAATCTAACCTAATTTCTTCAGTTC
CACAAATGGTCATCAATCCCTTACCATCCTCACTAGCACTAGACCATCTCTTATTACTTA
AATCCTCCTCAAAATCCTCACCCGTATCAGCAACTCTGCAACCAACACTGATGCTTAAAC
AATTCACCGCTCCCCTTGCAACCTCTTCATCTTCCACCACCTCAGTTTTATGAATTTTTT
TCTTCAACTTCTGGCTAACACTTTTCACAAGCCTCCTCGAAACGGTTAAAACCTCCAGAT
CCTGCCGTATAGATTCTTCTCCCAACGGTTTCTCAGAAATCATCATCCGTCATCTAACAA
ACTTCACAAGTCAGACACCTTGATCATGCTAAGAAGACCATCATTTTCCACAGATAATCA
ACCTGCAAATGCAAATCA
Primers used for PCR 46wd - 8474, show primer details
   
Sequence Name 37-K118262-0022-031-G10C-46wd
GenBank Accession LN502777 [GenBank]
Sequencing primer gene specific primer, 46wd: 5'-GCATGTCTCATAGACGCGATTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118262-0022-031-G10C-46wd
ATAGAACGACCTTCAACTATATAAATCTCCTCATGGATGCTTGTCACGGAGTACATAAAC
CNGGCCTTTAAGTAGATCAGTTTCAATCCTATGCCATTTGTCCTGAGAAACATCATACCC
GTGAATATCACCAGAGGAGCATCCATCTTTAAGAGCAGCAAACAGAAACAACCACGGTGT
TTGAAAGGAGCCTTCTCGTCTCATCTGGAGAAACCGCTGAGTGTTGGCCATGGATTGCCA
TTTCTTGCAAACAAGGTGTGCATTCAATAAGCTGGTCAAAGGAAGCCTCATCAAACACAT
TTCCAGAATATCATCGGGAAGATAAACGTGCTTGCGGTAATCTTCTATTATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 17-K118261-0022-031-G10C-oriL
GenBank Accession LN494053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118261-0022-031-G10C-oriL
TTACCGCAAGCACGTTTATCCTTCCCGAAGATATTCTGGAAATGTGTTTGATGAGGCTTC
CTTTGACCAGCTTATTGAATGCACACTCTTGTTTGCAAGAAATGGCAATCCATGGCCAAC
ACTCAGCGGTTTCTCCAGATGAGACGAGAAGGCTCCTTTCAAACACCGTGGTTGTTTCTG
TTTGCTGCTCTTAAAGATGGATGCTCCTCTGGTGATATTCACGGGTATGATGTTTCTCAG
GACAAATGGCATAGGATTGAAACTGATCTACTTAAAGGCCGGTTTATGTACTCCGTGACA
AGCATCCATGAGGAGATTTATATAGTTGGAGGTCGTTCTATGGACAGAAACTCTTTCAAG
TCCCACAGAGGGATCTTGGTATTCAGCCCTTCGATCAAAGCATGGCGTAAAATCGCG

Last Updated on 06.12.2018 16:11