Sequences from confirmation amplicon


Confirmation sequences for line 032A01 and hit At5g43140 are shown.
Line ID 032A01
Locus At5g43140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17323297
Primers used for PCR CW07 - 8474, show primer details
   
Sequence Name 33-K104113-0022-032-A01C-CW07
GenBank Accession LN500972 [GenBank]
Sequencing primer gene specific primer, CW07: 5'-TTTAAGAAAATAATGATGGGGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104113-0022-032-A01C-CW07
AGTCTTCATTCCTACATGCTGCTTTACAAGGTACATAACATAATCAAACCTCTATGGATT
GAACGGATTTAGCTTTTTACGGTTTCTCTGCCTGTAAATTTATGGTGCTCATTATGAGAT
TGGTTCTGGCACGTTGTTGGTCCTTTTTAAGGCGAAAATTCTGAGGAAATTGTGGCGAGA
TTGAAGAGAGATCTTCTGCCAACATTGAAAAATGGACTTATGTATTGGCCGGTCTGTGAT
TTTGTTACATTCAAGTATGTGCCAGTTCATCTACAGGTAAACCAAATATCTATCTGATTA
CTTTCTCTATTGTTTGGGATGTTTGAAGACACATTCTTAACTTCAACTTGTAATCTTCTG
GTTGCAGCCACTGATGAATAGCTCGTGCGCATATATATGGACAATCTATTTGACATATAT
GGCAAACCACACGAAAGCTGACAGCTGAACAATGACTAGTATGAGTGTTCTGGGTCACAC
GCAATCGCATTCTTTTTATTCCATTGAACCAACCATCAGTAGACTGGCTTCATGTC
   
Sequence Name 41-K104114-0022-032-A01C-8409
GenBank Accession LN504058 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K104114-0022-032-A01C-8409
CCGGAATGAGCCTTACTGATGGTTGGTTCAATTGAATAAAAAGAATGCGATTGCGTGTGA
CCCAGAACACTCATACTTGTCATTGTTCAGCTGTCAGCTTTCGTCTGGTTTGCCATATAT
GTCAAATAGATTGTCCATATATATGCGCACGAGCTATTCATCAGTGGCTGCAACCAGAAG
ATTACAAGTTGAAGTTAAGAAGTGTCTTCAAACATCCCAAACAATAGAGAAAGTAATAAG
ATAGATATTTGGTTTACCTGTAGATGAACTGGCACATACTTGAATGTAACAAAATCACAG
ACCGGCCAATACATAAGTCCATTTTTCAATGTTGGCAGAAGATCTCTCTTCAATCTCGCC
ACAATTTCCTCAGAATTTTCGCCTTAAAAAGGACCAACAACGTGCCAGAACCAATCTCAT
AATGAGCACCATAAATTTACAGGCAGAGAAACCGTAAAAAGCTAAATCCGTTCAATCCAT
AGAGGTTTGATTATGTTATGTA

Last Updated on 06.12.2018 16:11