Sequences from confirmation amplicon


Confirmation sequences for line 032C02 and hit At3g12670 are shown.
Line ID 032C02
Locus At3g12670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4021626
Primers used for PCR 09mm - 8474, show primer details
   
Sequence Name 36-K114362-0022-032-C02C-09mm
GenBank Accession LN502316 [GenBank]
Sequencing primer gene specific primer, 09mm: 5'-ATCAAATGAGAGAGCCAGATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114362-0022-032-C02C-09mm
CAGGGCTTGAGTTTCTGGTTCAACTCTGTGCTGTTTGCATCGTGAAAACCAAGAATGGAG
CGGGCAAATTCAACAACAGCCAGCTGCATTCCCAGGCATATGCCAAGGAAAGGGACTTGA
TTTTCACGGGCGTACTTTGTAGCAAGTATCTTCCCTTGCACTCCTCTATCACCAAACCCT
CCTGGTACTAGGATACCATCAGCACCCTGCAACAACCGAAGTGTTGAGTCAAACATATTT
ACCAAATTGGTGTGCCAGGCTCCATTAAAATTCACGTACCTTCAAAAGATCCCATGCAGC
CTTATGGACATCTGGCGTCTGCAAGGGTACGCTAAATCAATAATGGCTTCCCGAAGAATA
GATACCATCAATAATGGCGTCTGCATGGGCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 06-K114361-0022-032-C02C-8409
GenBank Accession LN488908 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114361-0022-032-C02C-8409
TTACATTGATATATCCTGCCCATGCAGACGCCATTATTGATGGTATCTATTCTTCGGGAA
GCCATTATTGATTTAGCGTACCCTTGCAGACGCCAGATGTCCATAAGGCTGCATGGGATC
TTTTGAAGGTACGTGAATTTTAATGGAGCCTGGCACACCAATTTGGTAAATATGTTTGAC
TCAACACTTCGGTTGTTGCAGGGTGCTGATGGTATCCTAGTACCAGGAGGGTTTGGTGAT
AGAGGAGTGCAAGGGAAGATACTTGCTACAAAGTACGCCCGTGAAAATCAAGTCCCTTTC
CTTGGCATATGCCTGGGAATGCAGCTGGCTGTTGTTGAATTTGCCCGCTCCATTCTTGGT
TTTCACGATGCAAACAGCACAGAGTTTGAACCAGAAACTTCAAGCCCTTGCATCATATTT
ATGCCAGAAGTACTAAACAATCTGGCTCTCTAATTTG

Last Updated on 06.12.2018 16:11