Sequences from confirmation amplicon


Confirmation sequences for line 032F01 and hit At2g46440 are shown.
Line ID 032F01
Locus At2g46440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19062459
Primers used for PCR UK12 - 8474, show primer details
   
Sequence Name 23-K019822-022-032-F01C-UK12
GenBank Accession LN496369 [GenBank]
Sequencing primer gene specific primer, UK12: 5'-GGGATCAAGAGCAAAGACTTACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K019822-022-032-F01C-UK12
AGCATGTAAAGGAAGAAGTTTAAAGCAGCTCCGACCCGCTTTGATTCTGCTACCGTACCA
AAGGCTCTTGTCACTTCTTTATAAAGCGGATACATACGAATGATTCTTGGTACATATTGA
CTAAGTATAATCCATTTCAGTATTCTCTCTGACACCAGCGACGCAGACAGTGGTATGAGA
GTGAGAACCACCACCTATAATTACGCATGTACATCTCTAATAAAAAGCATGGAAAAGTAG
ATGTCATGCACACACAAACGAAGGGAACAATACCTGAGGAATGGGGAGAACAGAGATAAT
GTCAACAATGAAGTGAAAGAGGAGACGTGTTTTCAGTGTAGCCTTAGAATGCACGACAAT
CTCGCCTCTCAAAGATGCTTGAGAACGAGGAGCAATGGTCTCGGTTATAAGATAATAAAT
GATGTGAATGGCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCT
   
Sequence Name 24-K019822-022-032-F01C-8409
GenBank Accession LN496811 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K019822-022-032-F01C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGCCATTCA
CATCATTTATTATCTTATAACCGAGACCATTGCTCCTCGTTCTCAAGCATCTTTGAGAGG
CGAGATTGTCGTGCATTCTAAGGCTACACTGAAAACACGTCTCCTCTTTCACTTCATTGT
TGACATTATCTCTGTTCTCCCCATTCCTCAGGTATTGTTCCCTTCGTTTGTGTGTGCATG
ACATCTACTTTTCCATGCTTTTTATTAGAGATGTACATGCGTAATTATAGGTGGTGGTTC
TCACTCTCATACCACTGTCTGCGTCGCTGGTGTCAGAGAGAATACTGAAATGGATTATAC
TTAGTCAATATGTACCAAGAATCATTCGTATGTATCCGCTTTATAAAGAAGTGACAAGAG
CCTTTGGTACGGTAGCAGAATCAAAGCGGGTCGGAGCTGCTTTAAACTTCTTCCTTTACA
TGCTGCACAGTTATGTAAGTCTTTGCTCTTGAT

Last Updated on 06.12.2018 16:11