Sequences from confirmation amplicon


Confirmation sequences for line 034C01 and hit At1g11720 are shown.
Line ID 034C01
Locus At1g11720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3953478
Primers used for PCR AK06 - 8474, show primer details
   
Sequence Name 13-K016839-022-034-C01C-8409
GenBank Accession LN491820 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K016839-022-034-C01C-8409
TAGATTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACCTTTTATTGTAATAT
AGCTTCACTGTATCTTCAGCCTTGAAATCACTCGGCTCAATGTACCAAACATTCTCAGCC
GAGACTACAGCTTTCTTAATAGCCGGTTGAAGCAATTCACGTCTCTTCTGAGTCTCCGCC
TTTGCTTGTGCTCTATCAGCTTCAATTGCAGCCTTTTGAGCTTCGATTCTTCTCTTCTCT
TCTTTTTGCCTCTCCCTCTCAGCTTCTTCCTTGGCTAACTTCTCTTGCTCTCGCAGTTTC
TCTTCTAGAAGAAAATTCTCAAAGTCAACTTTATCCATCCCACCTTTTATCTCTACACAA
AAATCTTTTGAGTCATTGTTGTCATATACACTTTGCCCATTGAAAAACACGAAGTCCATC
TTATACGCTTCTTTGGGGATATGAAGGAGACATGACAACCAATCTTCATGGATCCAGGTC
TTTTCCAATCTCCTT
   
Sequence Name 14-K016839-022-034-C01C-AK06
GenBank Accession LN492282 [GenBank]
Sequencing primer gene specific primer, AK06: 5'-AAGTCTTTCACAAGGAGATTGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K016839-022-034-C01C-AK06
CTGGATCCATGAAGATTGGTTGTCATGTCTCCTTCATATCCCCAAAGAAGCGTATAAGAT
GGACTTCGTGTTTTTCAATGGGCAAAGTGTATATGACAACAATGACTCAAAAGATTTTTG
TGTAGAGATAAAAGGTGGGATGGATAAAGTTGACTTTGAGAATTTTCTTCTAGAAGAGAA
ACTGCGAGAGCAAGAGAAGTTAGCCAAGGAAGAAGCTGAGAGGGAGAGGCAAAAAGAAGA
GAAGAGAAGAATCGAAGCTCAAAAGGCTGCAATTGAAGCTGATAGAGCACAAGCAAAGGC
GGAGACTCAGAAGAGACGTGAATTGCTTCAACCGGCTATTAAGAAAGCTGTAGTCTCGGC
TGAGAATGTTTGGTACATTGAGCCGAGTGATTTCAAGGCTGAAGATACAGTGAAGCTATA
TTACAATAAAAGGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11