Genotyping and primer details


Genotyping and primer details for 034C01 - At1g11720
Line ID 034C01
Locus At1g11720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3953478 of line 034C01 Chr1:3953478
   
Insertion allele amplicon 8474 - AK06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AK06: 5'-AAGTCTTTCACAAGGAGATTGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3953012
   
wt amplicon (over InsPos) FL63 - AK06
wt amplicon start Chr1:3953012
wt amplicon end Chr1:3953840
wt amplicon size 829 bp
Locus specific primer AK06: 5'-AAGTCTTTCACAAGGAGATTGGAA-3'
2nd locus specific primer FL63: 5'-CGTGGAAGTCTTGGTAACCATTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11