Sequences from confirmation amplicon


Confirmation sequences for line 034C09 and hit At5g47040 are shown.
Line ID 034C09
Locus At5g47040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19096002
Primers used for PCR CP33 - 3144, show primer details
   
Sequence Name 41-K031137-022-034-C09C-CP33
GenBank Accession LN503966 [GenBank]
Sequencing primer gene specific primer, CP33: 5'-ACCATCCAACATGACTGTAATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K031137-022-034-C09C-CP33
GAGCTTCTACTAGGCAGTTTTGCCAATTCCTGTTCAGAAAAGCTAAGTAGCAATCATTTT
GATGATCTATAACACTTTCTGTCACTTATACTTGTCAAGGACAGACAGAAACTTATCTGC
TACAACTTAGTTCCTGTCCTAAAACAACTCCCCAAGTTCCTCATCAGCATAAGCTAGTTA
CGTATTCTTAGAAAACAAGACTCACCTTTAGGCCATCAATGAGGCGCCCTGGCATGCTTC
CTATATAAGTTCTTCTGTGTCCTCTAATGTCAGCTTCATCTTTCACACCACCCAAAGATA
AGCGAACAAATTTTCTACCTAATGCAGCTGCAATAGATGATGCCAAAGACGTCTTGCCCA
CACCTGGTGGACCAACAAAGCACAATACTGGGCCTGAAAGAATATGATGACCAACAAACA
CTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGG
AACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
   
Sequence Name 06-K031137-022-034-C09C-3144
GenBank Accession LN488687 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K031137-022-034-C09C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGTT
GGTCATCATATTCTTTCAGGCCCAGTATTGTGCTTTGTTGGTCCACCAGGTGTGGGCAAG
ACGTCTTTGGCATCATCTATTGCAGCTGCATTAGGTAGAAAATTTGTTCGCTTATCTTTG
GGTGGTGTGAAAGATGAAGCTGACATTAGAGGACACAGAAGAACTTATATAGGAAGCATG
CCAGGGCGCCTCATTGATGGCCTAAAGGTGAGTCTTGTTTTCTAAGAATACGTAACTAGC
TTATGCTGATGAGGAACTTGGGGAGTTGTTTTAGGACAGGAACTAAGTTGTAGCAGATAA
GTTTCTGTCTGTCCTTGACAAGTATAAGTGACAGAAAGTGTTATAGATCATCAAAATGAT
TGCTACTTAGCTTTTCTGAACAGGAATTGGCAAAACTGCCTAGTAGAAGCTCTTATGATA
TGTGATTGTCCAGTTTGATTACAGTCTGTTGGGGAAG
Primers used for PCR AF07 - 3144, show primer details
   
Sequence Name 18-K105138-0022-034-C09C-AF07
GenBank Accession LN494301 [GenBank]
Sequencing primer gene specific primer, AF07: 5'-ACCATCCAACATGACTGTAATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K105138-0022-034-C09C-AF07
GCAGTTTTGCCATTCCTGTTCAGAAAAGCTAAGTAGCAATCATTTTGATGATCTATAACA
CTTTCTGTCACTTATACTTGTCAAGGACAGACAGAAACTTATCTGCTACAACTTAGTTCC
TGTCCTAAAACAACTCCCCAAGTTCCTCATCAGCATAAGCTAGTTACGTATTCTTAGAAA
ACAAGACTCACCTTTAGGCCATCAATGAGGCGCCCTGGCATGCTTCCTATATAAGTTCTT
CTGTGTCCTCTAATGTCAGCTTCATCTTTCACACCACCCAAAGATAAGCGAACAAATTTT
CTACCTAATGCAGCTGCAATAGATGATGCCAAAGACGTCTTGCCCACACCTGGTGGACCA
ACAAAGCACAATACTGGGCCTGAAAGAATATGATGACCAACAAACACTGATAGTTTAAAC
CGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAAC
TTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGT
CAGTGGAGATATCA
   
Sequence Name 02-K105139-0022-034-C09C-3144
GenBank Accession LN487028 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K105139-0022-034-C09C-3144
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGTTGGTCATCATAT
TCTTTCAGGCCCAGTATTGTGCTTTGTTGGTCCACCAGGTGTGGGCAAGACGTCTTTGGC
ATCATCTATTGCAGCTGCATTAGGTAGAAAATTTGTTCGCTTATCTTTGGGTGGTGTGAA
AGATGAAGCTGACATTAGAGGACACAGAAGAACTTATATAGGAAGCATGCCAGGGCGCCT
CATTGATGGCCTAAAGGTGAGTCTTGTTTTCTAAGAATACGTAACTAGCTTATGCTGATG
AGGAACTTGGGGAGTTGTTTTAGGACAGGAACTAAGTTGTAGCAGATAAGTTTCTGTCTG
TCCTTGACAAGTATAAGTGACAGAAAGTGTTATAGATCATCAAAATGATTGCTACTTAGC
TTTTCTGAACAGGAATTGGCAAAACTGCCTAGTAGAAGCTCTTATGATATGTGATTGTCC
AGTTTGATTACAG
Primers used for PCR AG13 - 3144, show primer details
   
Sequence Name 42-K105356-0022-034-C09C-AG13
GenBank Accession LN504461 [GenBank]
Sequencing primer gene specific primer, AG13: 5'-ACCATCCAACATGACTGTAATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K105356-0022-034-C09C-ag13
GCAGTTTTGCCATTCCTGTTCAGAAAAGCTAAGTAGCAATCATTTTGATGATCTATAACA
CTTTCTGTCACTTATACTTGTCAAGGACAGACAGAAACTTATCTGCTACAACTTAGTTCC
TGTCCTAAAACAACTCCCCAAGTTCCTCATCAGCATAAGCTAGTTACGTATTCTTAGAAA
ACAAGACTCACCTTTAGGCCATCAATGAGGCGCCCTGGCATGCTTCCTATATAAGTTCTT
CTGTGTCCTCTAATGTCAGCTTCATCTTTCACACCACCCAAAGATAAGCGAACAAATTTT
CTACCTAATGCAGCTGCAATAGATGATGCCAAAGACGTCTTGCCCACACCTGGTGGACCA
ACAAAGCACAATACTGGGCCTGAAAGAATATGATGACCAACAAACACTGATAGTTTAAAC
CGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAAC
TTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGT
CAGTGGAGATA
   
Sequence Name 25-K105357-0022-034-C09C-3144
GenBank Accession LN497439 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K105357-0022-034-C09C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATAAAGGAAGTTCATTTCATTTGGACAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGTTG
GTCATCATATTCTTTCAGGCCCAGTATTGTGCTTTGTTGGTCCACCAGGTGTGGGCAAGA
CGTCTTTGGCATCATCTATTGCAGCTGCATTAGGTAGAAAATTTGTTCGCTTATCTTTGG
GTGGTGTGAAAGATGAAGCTGACATTAGAGGACACAGAAGAACTTATATAGGAAGCATGC
CAGGGCGCCTCATTGATGGCCTAAAGGTGAGTCTTGTTTTCTAAGAATACGTAACTAGCT
TATGCTGATGAGGAACTTGGGGAGTTGTTTTAGGACAGGAACTAAGTTGTAGCAGATAAG
TTTCTGTCTGTCCTTGACAAGTATAAGTGACAGAAAGTGTTATAGATCATCAAAATGATT
GCTACTTAGCTTTTCTGAACAGGAATTGGCAAAACTGCCTAGTAGAAGCTCTTATGATAT
GTGATTGTCCAGTTTGATTACAGT

Last Updated on 06.12.2018 16:11