Genotyping and primer details


Genotyping and primer details for 034C09 - At5g47040
Line ID 034C09
Locus At5g47040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19096002 of line 034C09 Chr5:19096002
   
Insertion allele amplicon 3144 - AF07
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AF07: 5'-ACCATCCAACATGACTGTAATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19095562
   
Insertion allele amplicon 3144 - AG13
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AG13: 5'-ACCATCCAACATGACTGTAATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19095562
   
Insertion allele amplicon 3144 - CP33
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CP33: 5'-ACCATCCAACATGACTGTAATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19095562
   
wt amplicon (over InsPos) AL08 - AG13
wt amplicon start Chr5:19095562
wt amplicon end Chr5:19096613
wt amplicon size 1052 bp
Locus specific primer AG13: 5'-ACCATCCAACATGACTGTAATCAA-3'
2nd locus specific primer AL08: 5'-CTGGTTGTCAACTTGAGTCTTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11