Sequences from confirmation amplicon


Confirmation sequences for line 034E11 and hit At5g18600 are shown.
Line ID 034E11
Locus At5g18600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6183958
Primers used for PCR 13nc - 8474, show primer details
   
Sequence Name 71-K114351-0022-034-E11C-13nc
GenBank Accession LN512601 [GenBank]
Sequencing primer gene specific primer, 13nc: 5'-ATATCACCATGCCAAGATCATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114351-0022-034-E11C-13nc
AACATGATAAGTCTAACCCACAATGCACCAGCCCGCTTAAGCATGGGAATCAAGGATCCG
TTAAGATGTAGACTCATGACCCTCGTTGGCTCCACCGACCAACTCTCCACCAATGAAAAC
GCCCGGAACTGCGGGGCTACACCCGAGCCGCAACAACGCCTGCTCGATCTCCCTCCCTCT
AGATATCTCATCCAGCTCGTAAACCGCTGGATTTGCTCCGAAATCGCAGAGCAAAGTCTT
GATCGTGTGAGACATACAGCAAGAGCTCTTGCTGTAAATCACCACCGGTCTCTCCATCAC
CATCTTCGTTATCATATCCATTGCTATTTTATATGGTATATGATCCAACAGCTTCTTAAG
TCCTTTCTTAAATAGGTCTATGAATCTATATTTATGATCTCTTAGTATATAGATCGATTT
TTTTTTTGTAAAAATTTAAGGAAAAAAACTACTCTTGATTCTCCGTGGAAAATAAGGGTA
TTGGGAAAACGTTGGGTGGATATATAAAAAAGGGG
   
Sequence Name 23-K114350-0022-034-E11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114350-0022-034-E11C-8409
GAATCTCGTAACCTGAAATTCGGATTTCCC

Last Updated on 06.12.2018 16:11