Sequences from confirmation amplicon


Confirmation sequences for line 034H01 and hit At4g12440 are shown.
Line ID 034H01
Locus At4g12440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7378163
Primers used for PCR RZ07 - 8474, show primer details
   
Sequence Name 57-K017462-022-034-H01C-RZ07
GenBank Accession LN509265 [GenBank]
Sequencing primer gene specific primer, RZ07: 5'-GGGACATTGGAACTAAACCACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K017462-022-034-H01C-RZ07
ATATATGTTTCTATAGTTTCTAATATACGCATGAGCCGTTTCATACTATTTGAAGACTGT
GTATTTTTGTTGTTTTAATCAAACACATATTTGATATATTTTAACCATGGACTAACAGTA
ATTTTTTTTTATGAAAAACAATGCTATATTCGGACCATATTTTCATGTGTAAAGAATTTT
GATGTCATTGTAACTGTCGATGTTTATAACGATCGATCATATAAGAAATTTTACCGCATA
CACACAAAAAGGTAGTTTCAAGGGACTGAGATATTAAGAAATTTCATTTTATTTTATTTT
TGATAAAAGAATTGCATATATTTCAAGTATAAATCCATGTAAACTTGGTCCTTTGTATCA
TCGTAAGTTGGCATGTGCTCTTGAACCCGTCTCTTGCGTATGTTACAAGGACAAAAAAAG
AGAGCCTCGTTTGTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTC
CTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAATCCTTTACAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAA
   
Sequence Name 58-K017462-022-034-H01C-8409
GenBank Accession LN509570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K017462-022-034-H01C-8409
TGTAGATTTCCCGGAATGAAGCCCTTTACAATTGAATATATCCTGTAAAGGATTTCGCAA
GACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATC
AGATTGTCGTTTCCCGCCTTCGGTTTAACAAACGAGGCTCTCTTTTTTTGTCCTTGTAAC
ATACGCAAGAGACGGGTTCAAGAGCACATGCCAACTTACGATGATACAAAGGACCAAGTT
TACATGGATTTATACTTGAAATATATGCAATTCTTTTATCAAAAATAAAATAAAATGAAA
TTTCTTAATATCTCAGTCCCTTGAAACTACCTTTTTGTGTGTATGCGGTAAAATTTCTTA
TATGATCGATCGTTATAAACATCGACAGTTACAATGACATCAAAATTCTTTACACATGAA
AATATGGTCCGAATATAGCATTGTTTTTCATAAAAAAAAATTACTGTTAGTCCATGGTTA
AAATATATCAAATATGTGTTTGATTAAAACAACAAAAATACACAGTCTTCAAATAGTATG
AAACGGCCATGCGTATATTAGAAACTATAGAAACATATATGACTACAAATGTT
Primers used for PCR FL66 - 3144, show primer details
   
Sequence Name 31-K029300-022-034-H01C-3144
GenBank Accession LN500030 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K029300-022-034-H01C-3144
ATCCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGG
ACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGTTTTTTCTTTTGTTAAAGTC
AATACTTTCCACTTAAAGTCGAGCCTTTACAAAAATGTTTTCAAGGAATCTTTTAACAGT
CGGGGAATTAGAGTTCTCATGTCTTTTTCATATTGATTTAATTGCTAATATATATATATA
TATACACGACTGATTTTTTTGTTTTCCTTACGTTTTGCTTGTTTATAGGTATAGAGGCTC
GCGGTTTCATATTCGGTTCACCCATCGCATTAGCCATCGGAGCAAAATTTGTTCCTCTTC
GTAAACCAAAGAAATTGCCGGGTTAAACACCATTCCAAACTTTTATATTTAACATATGCT
TTGTTATTTTATTATTCTTATATGTCAAGCGATATTTTGAGTAACAATAGTGTTACCTAT
CGTTTAACAAATCAAGTATGGGGTTTAATTATCAAAAAAAGTATGGGGTTTAACTATACG
ATTTTTAATCAGGCCAAATAATATTTGAAGAGTATGAGTTGGAGTACAGAAGTGACCGGT
TGGAAATGCATGTTGAAGCTGTAGATTCCGGAGATAGAGCATTGGTCGTAGATGATCAGA
TTGCTACCGGCGGGACTCTNTGTGCTGCCATGAATTTGCTCACTAAATTTTTCTCATTTT
TCAACAATATATCTATAGTACCAAAATTATTATTATCTTTCAAATGGCTGCCCATTGATT
CANATCATTT
   
Sequence Name 32-K029300-022-034-H01C-FL66
GenBank Accession LN500453 [GenBank]
Sequencing primer gene specific primer, FL66: 5'-GGATTTATTGAAGTGGCAAATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K029300-022-034-H01C-FL66
ATAATAATTTTGGTACTAAAGATATANTTGTTGAAAAATGAGAAAAATTTACTGAGCAAA
TTCATGGCAGCACAAAGAGTCCCGCCGGTAGCAATCAGATCATCTACGACCAATGCTCTA
TCACCGGAATCTACAGCTTCAACATGCATTTCCAACCGGTCACTTCCGTACTCCAACTCA
TACTCTTCAAATATTATTTGGCCTGATTAAAAATCGTATAGTTAAACCCCATACTTTTTT
TGATAATTAAACCCCATACTTGATTTGTTAAACGATAGGTAACACTATTGTTACTCAAAA
TATCGCTTGACATATAAGAATAATAAAATAACAAAGCATATGTTAAATATAAAAGTTTGG
AATGGTGTTTAACCCGGCAATTTCTTTGGTTTACGAAGAGGAACAAATTTTGCTCCGATG
GCTAATGCGATGGGTGAACCGAATATGAAACCGCGAGCCTCTATACCTATAAACAAGCAA
AACGTAAGGAAAACAAAAAAATCAGTCGTGTATATATATATATATATTAGCAATTAAATC
AATATGAAAAAGACATGAGAACTCTAATTCCCCGACTGTTAAAAGATTCCTTGAAAACAT
TTTTGTAAAGGCTCGACTTTAAGTGGAAAGTATTGACTTTAACAAAAGAAAAAACGAAGG
CGGGAAACGACAATCTGATCCCCGGGTACTCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATA

Last Updated on 06.12.2018 16:11