Sequences from confirmation amplicon


Confirmation sequences for line 035F02 and hit At1g11810 are shown.
Line ID 035F02
Locus At1g11810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3990827
Primers used for PCR 17nc - 8474, show primer details
   
Sequence Name 68-K114351-0022-035-F02C-17nc
GenBank Accession LN512063 [GenBank]
Sequencing primer gene specific primer, 17nc: 5'-TGACTTCACAAGAGAGAGTTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K114351-0022-035-F02C-17nc
GCATTATGGTTATATCTTGTGTTAGAGATGATCATCTTGCTGTGTTATTCAACGTGACAC
TTTAGGTATTTGGATTTCGACTGAGATCGAGCCCAACAAGGTGTCGTGGAGAGAGTTTTT
GCAAGTGGACTTGGCGACGCTGGATGGTTTTCCGGATGTATTTATAGCTGGGAGATTCAT
CGTTGACGAGGAGAAGCAAGTCGTTGTAGTTTTCGGTCAAGAGACAGAGTTGGATCTCAA
CCACGGAAATGCTTTCATCTTTGGAAGAGATGGATACTTCACCTCTTTTACCGTTGGAGA
TGCTCCGCCCGAAGACTTCACTTCTTATGTTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCACGATTATTA
   
Sequence Name 20-K114350-0022-035-F02C-8409
GenBank Accession LN495307 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114350-0022-035-F02C-8409
GAGTGAGTCTTCGGGCGGAGCATCTCCACGGTAAAAGAGGTGAAGTATCCATCTCTTCCA
AAGATGAAAGCATTTCCGTGGTTGAGATCCAACTCTGTCTCTTGACCGAAAACTACAACG
ACTTGCTTCTCCTCGTCAACGATGAATCTCCCAGCTATAAATACATCCGGAAAACCATCC
AGCGTCGCCAAGTCCACTTGCAAAAACTCTCTCCACGACACCTTGTTGGGCTCGATCTCA
GTCGAAATCCAAATACCTAAAGTGTCACGTTGATATAACACAGCAAGATGATCATCTCTA
ACACAAGATATAACCATAATGCCTTCTCCTTCATCACCCAAAGACTCAAACGGCAGAGAC
AGAAGCTTTCCAAAACTCTCTCTGNTGAAGTC

Last Updated on 06.12.2018 16:11