Sequences from confirmation amplicon


Confirmation sequences for line 036A05 and hit At1g60470 are shown.
Line ID 036A05
Locus At1g60470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22279559
Primers used for PCR 23mm - 8474, show primer details
   
Sequence Name 55-K114263-0022-036-A05C-23mm
GenBank Accession LN508880 [GenBank]
Sequencing primer gene specific primer, 23mm: 5'-AGATATCTCAAGCAGCAGAAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114263-0022-036-A05C-23mm
GACGGAGCTAGGATGCTTGATTTTGTAACGGTTTCTTCCGCATCTGCAGGGATTTTCGAT
TTGAAATCGAGAGATTCATCATTATAGACATCCCACCATTTATCAACTAACATCTTGATG
TCTTCTCTGTCCATGTTAGCTTCTTCTCCTGTGTATCTCCATGGCTTGGATCCCTATCAA
CAGAAAAAAGGAATCAAATTTCAGCAACAAGCTTAAGGGGCTCAGGGTTTCAAAGCAAAG
ACAGAACTACTTACAGCTGCACAGTAGTGAACAACTTTGACTTTCTCCAGCTCCACGTTC
TCCGGGTGACGCCAAAGCATAGCCAAAACCAGATTGTATACCAAAGGGATCGGCAATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 12-K114262-0022-036-A05C-8409
GenBank Accession LN491673 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114262-0022-036-A05C-8409
TCTGGTTTTGGCTTGCTTTGGCGTCACCCGGAGAACGTGGAGCTGGAGACATCAAAGTTG
TTCACTACTGTGCAGCTGTAAGTAGTTCTGTCTTTGCTTTGAAACCCTGAGCCCCTTAAG
CTTGTTGCTGAAATTTGATTCCTTTTTTCTGTTGATAGGGATCCAAGCCATGGAGATACA
CAGGAGAAGAAGCTAACATGGACAGAGAAGACATCAAGATGTTAGTTGATAAATGGTGGG
ATGTCTATAATGATGAATCTCTCGATTTCAAATCGAAAATCCCTGCAGATGCGGAAGAAA
CCGTTACAAAATCAAGCATCCTAGCTTCCGTTCTTGAACCAGAAATGACCTACTTTCCTG
CTCCTTCTGCTGCTTGAGATATC

Last Updated on 06.12.2018 16:11