Sequences from confirmation amplicon


Confirmation sequences for line 036C10 and hit At1g65570 are shown.
Line ID 036C10
Locus At1g65570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24375307
Primers used for PCR 37os - 8474, show primer details
   
Sequence Name 09-K114815-0022-036-C10C-37os
GenBank Accession LN490299 [GenBank]
Sequencing primer gene specific primer, 37os: 5'-GAGGTTAGGTTCGAAGGAGAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114815-0022-036-C10C-37os
GACTTTATTGGTCCTCAAGACTACAGTTTGCTTGGGAAAAAGAAAACTGGTTTAGTTTTA
GTGGTGTTCACAATGTTACGGTTCTTGGAGGTTCTTTTGATGCTAAGGGATCAACCTTGT
GGAGTTGCAAGGCTAATGGTTACAACTGCCCTGAAGGTGCAACGGTATGTGTTTAAGTCT
TCAATTACCAATTTATGCATTTGTGTTTTTCTTTTTTGTCAACAACATGAACACAACTAC
ACTTGCATTTTTTTTTCTGTTATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 01-K114814-0022-036-C10C-8409
GenBank Accession LN486686 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K114814-0022-036-C10C-8409
GAAAAAAAATGCAAGTGTAGTTGTGTTCATGTTGTTGATAAAAAAGAAAAACACAAATGC
ATAAATTGGTAATTGAAGACTTAAACACATACCGTTGCACCTTCAGGGCAGTTGTAACCA
TTAGCCTTGCAACTCCACAAGGTTGATCCCTTAGCATCAAAAGAACCTCCAAGAACCGTA
ACATTGTGAACACCACTAAAACTAAACCAGTTTTCTTTTTTCCCAAGCAAACTGTAGTCT
TGAGGACCAATTAAAGTTCCGTCAATCCTTAGGGTTATCTCACGGCTTTTGCAACTTTCT
CCTTCGAACCTAACCT

Last Updated on 06.12.2018 16:11