Sequences from confirmation amplicon


Confirmation sequences for line 037C01 and hit At2g47130 are shown.
Line ID 037C01
Locus At2g47130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19349847
Primers used for PCR 17nh - 8474, show primer details
   
Sequence Name 06-K114392-0022-037-C01C-8409
GenBank Accession LN488909 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114392-0022-037-C01C-8409
AGTTTTTCTTACGCCGTTGACTCTATCCCGTACTTCCCTAGCCCGCCACAAGCCGATTTA
ACCAGCCCGAGAAGAGCGTGCTTAGACGCCGTGTACGCGTGAGGTCCTGGACCACCGATC
TCCGACGCGACGCTGGTCGTACATACGATTGACCCACGCGTGCCTTTCTCCACCATGGCT
CGTGCAGCGTGTTTGATAAACGCAGCCGCACCACGAACGTTGACCGCCATGGTTCGGTCA
AACTGTTCCAGATTCAAGTCGAGAAAGCTTCCCGGCTGTTCCATAACGCCGGCGTTACTA
AAGAGAACGTCAAGCTTCCCGTACTTTTCGACGGTGAACTTAACGGCGTTTTCTACCTCC
TTTTCGTTTGTAACATCGCAACGGTNAAAACTTGCTTTGTCTTT
   
Sequence Name 30-K114393-0022-037-C01C-17nh
GenBank Accession LN499822 [GenBank]
Sequencing primer gene specific primer, 17nh: 5'-GAAAGACAAAGCAAGTTTTTACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114393-0022-037-C01C-17nh
ACGCCGTTAGTTCACCGTCGAAAGTACGGGAAGCTTGACGTTCTCTTTAGTAACGCCGGC
GTTATGGAACAGCCGGGAAGCTTTCTCGACTTGAATCTGGAACAGTTTGACCGAACCATG
GCGGTCAACGTTCGTGGTGCGGCTGCGTTTATCAAACACGCTGCACGAGCCATGGTGGAG
AAAGGCACGCGTGGGTCAATCGTATGTACGACCAGCGTCGCGTCGGAGATCGGTGGTCCA
GGACCTCACGCGTACACGGCGTCTAAGCACGCTCTTCTCGGGCTGGTTAAATCGGCTTGT
GGCGGGCTAGGGAAGTACGGGATTAGAGTCAACGGCGTTAAGAAAAACTTACATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11