Sequences from confirmation amplicon


Confirmation sequences for line 037C06 and hit At1g17770 are shown.
Line ID 037C06
Locus At1g17770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6121074
Primers used for PCR CM19 - 8474, show primer details
   
Sequence Name 38-K031137-022-037-C06C-CM19
GenBank Accession LN502875 [GenBank]
Sequencing primer gene specific primer, CM19: 5'-AACCTTTGAATAATGTTCACTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K031137-022-037-C06C-CM19
AATTATGACTGATCTCTAACTTCTTCTTTTTGTATATAATGATAGGATTTTTTTTCCAAT
GGATAAGTCTATTCCAATCAAGGCAATACCGGTTGCATGTGTCAGACCAGATTTGGTAGA
TGACGTGACCAAAAACACATCAACGATTCCTACAATGGTTTCACCAGTTCTAACCAATAT
GCCATCTGCAACATCTCCTCTTCTAATGGTCCCACCTCTTCGAACAATCTGGCCATCCAA
CAAGGAATGGTACGATGGAGATGCTGGTCCTAGTAGTACTGGTCCAATCAAACGAGAAGC
GTCCGATAATACTAATGATACAGCACACAACACATTTGCACCTCCTCCAGAAATGGTCAT
ACCACTGATCACCATTAGGCCAAGTGATGACTTCAATTGTAAATGTGTTTAAACTATCAG
TGTTTAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGA
CGTGTCTACATTC
   
Sequence Name 03-K031137-022-037-C06C-8409
GenBank Accession LN487361 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K031137-022-037-C06C-8409
GAGCATTTAAATTTAACACTGATAGTTTAAACACATTTACAATTGAAGTCATCACTTGGC
CTAATGGTGATCAGTGGTATGACCATTTCTGGAGGAGGTGCAAATGTGTTGTGTGCTGTA
TCATTAGTATTATCGGACGCTTCTCGTTTGATTGGACCAGTACTACTAGGACCAGCATCT
CCATCGTACCATTCCTTGTTGGATGGCCAGATTGTTCGAAGAGGTGGGACCATTAGAAGA
GGAGATGTTGCAGATGGCATATTGGTTAGAACTGGTGAAACCATTGTAGGAATCGTTGAT
GTGTTTTTGGTCACGTCATCTACCAAATCTGGTCTGACACATGCAACCGGTATTGCCTTG
ATTGGAATAGACTTATCCATTGGAAAAAAAATCCTATCATTATATACAAAAAGAAGAAGT
TAGAGATCAGTCATAATTAATCAAGTATAGATATTATATATGGTATAGCGAGGAGATTTA
CGAAACATGGCAGTGAACATTATTCAAAGGTTAACTCCCATTTGGACGTGAATGTAGACA
CCCCATTTGGACGTGAATTTAAACCCCCCATTAAGCCCCGAATG

Last Updated on 06.12.2018 16:11