Sequences from confirmation amplicon


Confirmation sequences for line 037C07 and hit At1g72420 are shown.
Line ID 037C07
Locus At1g72420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27262258
Primers used for PCR 18nh - 8474, show primer details
   
Sequence Name 04-K114479-0022-037-C07C-8409
GenBank Accession LN488013 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114479-0022-037-C07C-8409
ATTTATATTGAATATTTGGTAATTTCTCTTAATCGAGCCTCAGGCGAAAGTATGAAGTTT
CTTGCTTCTCATACCATTTACCTCTCTTCATCATGTTTTTTTCTGCAATTGGGTGGTGAC
CGTTTTCACCTTATGTCTCCTTTAGGTGTTTTCTCTGGGAACCTCTCTACGGATATGAGG
GAAGGTTCAAAATGGAATATCAATCGGAGTGGTTTCTGTGGAATGCGGTCAAAGAAGGTT
AAAATCATAGGTTTTGTTTTTCTTTACTAATCATCACATCATCAATCAAAACCAAAGATT
TGCATGAGTTTATGCACAACTTACTTAACTAAAACCCCCTCCCATTTTTGTAGTTTGATG
GCTTTATTGATCTCGAAGGGTATGATTCCATAGCCCTGAGGCTCAAAGGAGATGGAAGGT
GCTATATTTCTACTGTGAGTCTCATGCTCAACATTCATGTCTT
   
Sequence Name 20-K114480-0022-037-C07C-18nh
GenBank Accession LN495312 [GenBank]
Sequencing primer gene specific primer, 18nh: 5'-AAAGACATGAATGTTGAGCATGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114480-0022-037-C07C-18nh
TCATCTCCTTTGACCTCGGGCTATGGAATCATACCCTTCGAGATCAATAAAGCCATCAAA
CTACAAAAATGGGAGGGGGTTTTAGTTAAGTAAGTTGTGCATAAACTCATGCAAATCTTT
GGTTTTGATTGATGATGTGATGATTAGTAAAGAAAAACAAAACCTATGATTTTAACCTTC
TTTGACCGCATTCCACAGAAACCACTCCGATTGATATTCCATTTTGAACCTTCCCTCATA
TCCGTAGAGAGGTTCCCAGAGAAAACACCTAAAGGAGACATAAGGTGAAAACGGTCACCA
CCCAATTGCAGAAAAAAACATGATGAAGAGAGGTAAATGGTATGAGAAGCAAGAAACTTC
ATACTTTCGCCTGAGGCTCGATTAAGAGAAATTACCAATATATTCAATTATAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTACGCAACGTTATA

Last Updated on 06.12.2018 16:11