Sequences from confirmation amplicon


Confirmation sequences for line 037C10 and hit At2g27940 are shown.
Line ID 037C10
Locus At2g27940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11898719
Primers used for PCR ML01 - 8474, show primer details
   
Sequence Name 19-K023019-022-037-C10C-8409
GenBank Accession LN494584 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K023019-022-037-C10C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCTCTGCCCCAAACTT
GAACAGCTACTACACCTCCTTACACAAGTGTCCACACCATCACACGTGTCATGTTCTTCT
GCCGAGTCCTGATCAGGCGGTTCTTGCATGCCCAAGTCCTTATCCGAGAACAGCTGGTTA
CTCCGGCACAAGGGACATGTTACGTATGAGCTCAGCCACGTGTCAACGCAATCTACGTGG
AACACGTGACCACAATGTGGTATCACTTTAACTGTTTCACCTTCTTCGAAATCGCTCAAG
CAGATAACGCAATCTTCGTTCCGCTGCTTCGCTGCTTTCGTGTAACGGTACACTGGCAGA
GATCGAACGGCCTGAAATGTCCCAATCTTGCCGGTTAACATAAGAACCGAGAAAGATAAT
TAGAGAAATGGATTTACCTGAGAATCAAGACCCCTCCGGAAACTATACGGCCGTGATACG
ACGACGGATGTGGAAAGGCGACGTGGAGACATCCTAGAGGATCTTGTTCTTGGCATGGAA
GAAATATCAACCGTTGAACTTGAACTGTCTGCGAAGTGGCGGAAGTAAACTGAAAAGAA
   
Sequence Name 20-K023019-022-037-C10C-ML01
GenBank Accession LN495040 [GenBank]
Sequencing primer gene specific primer, ML01: 5'-CTTCTTCATGGGTTTCTTTTCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K023019-022-037-C10C-ML01
TTCCGCCACTTCGCAGACAGTTCAAGTTCAACGGTTGATATTTCTTCCATGCCAAGAACA
AGATCCTCTAGGATGTCTCCACGTCGCCTTTCCACATCCGTCGTCGTATCACGGCCGTAT
AGTTTCCGGAGGGGTCTTGATTCTCAGGTAAATCCATTTCTCTAATTATCTTTCTCGGTT
CTTATGTTAACCGGCAAGATTGGGACATTTCAGGCCGTTCGATCTCTGCCAGTGTACCGT
TACACGAAAGCAGCGAAGCAGCGGAACGAAGATTGCGTTATCTGCTTGAGCGATTTCGAA
GAAGGTGAAACAGTTAAAGTGATACCACATTGTGGTCACGTGTTCCACGTAGATTGCGTT
GACACGTGGCTGAGCTCATACGTAACATGTCCCTTGTGCCGGAGTAACCAGCTGTTCTCG
GATAAGGACTTGGGCATGCAAGAACCGCCTGATCAGGACTCGGCAGAAGAACATGACACG
TGTGATGGTGTGGACACTTGTGTAAGGAGGTGTAGTAGCTGTTCAAGTTTGGGGCAGAGG
ACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
Primers used for PCR FL76 - 8474, show primer details
   
Sequence Name 55-K029745-022-037-C10C-8409
GenBank Accession LN508701 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K029745-022-037-C10C-8409
TTTACAATTGAATATATCCTATTCAATTGAATATACTCATTGGATTGGTGATGACATGTT
CTTTTTTTTTTGTGTTGTTCTTAATCTTGATTTTGTCTTTGTATTATTGTTGTAATAAGG
GAAGGGTATGAAATAATGACTTTGTAAAATGAGATTGAGTAGTAAATTTTGTTAGTGTTG
CCAAAATATTATTTTATTCATTCCATTTTGTTTTATGAGATGTTTAAGAATAAAACAAAA
TTATGAGTTTCTGATTTTGTATTACTATTTTCCGTGGAAGGAAACGGAAAAATTGAAGCG
AAATGTGTGGAACGNAAAATT
   
Sequence Name 56-K029745-022-037-C10C-FL76
GenBank Accession LN508997 [GenBank]
Sequencing primer gene specific primer, FL76: 5'-AAAAATTTCGTTTCCACACATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K029745-022-037-C10C-FL76
TAGTAATACAAAATCAGAAACTCATAATTTTGTTTTATTCTTAAACATCTCATAAAACAA
AATGGAATGAATAAAATAATATTTTGGCAACACTAACAAAATTTACTACTCAATCTCATT
TTACAAAGTCATTATTTCATACCCTTCCCTTATTACAACAATAATACAAAGACAAAATCA
AGATTAAGAACAACACAAAAAAAAAAGAACATGTCATCACCAATCCAATGAGTATATTCA
ATTGAATAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11