Sequences from confirmation amplicon


Confirmation sequences for line 037D07 and hit At5g60140 are shown.
Line ID 037D07
Locus At5g60140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24214549
Primers used for PCR 31mm - 8474, show primer details
   
Sequence Name 15-K115021-0022-037-D07C-31mm
GenBank Accession LN493095 [GenBank]
Sequencing primer gene specific primer, 31mm: 5'-TCCTCTTAAGCTCGCATATCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115021-0022-037-D07C-31mm
ATCCCTTGACAAAGATGCAACCATTCTTTTGAATCTTGATTGCTTTCTCCAATGCTCCCA
TTTCGTCCACGACTGTTATACGCTCCGGGAAGGTTAAGTCATAGTCCCTTATGACATGAG
CCGGTATGTGCTGCATCAAGAAACAAACATCATAAAACAGATTGAGATATTCCTTATTAA
TTTATTCACATAAGAATCCATAACCAAAGATGTTAAATGGAGAGTGTGTAAAGATCTTAA
ATATTCAGGTTTTTTTTTCTCTTACCAATTGGCTCTTCTTCTTCGGATTGAGTGTCATAG
TAAAGTACGGGTTCTCGTGATCATCCAGATACCGCCTTTCGTCATCCTCATCCTCAATTG
TAAATTTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCA
   
Sequence Name 02-K115020-0022-037-D07C-8409
GenBank Accession LN487153 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115020-0022-037-D07C-8409
AATTTACATTGAGGATGAGGATGACGAAAGGCGGTATCTGGATGATCACGAGAACCCGTA
CTTTACTATGACACTCAATCCGAAGAAGAAGAGCCAATTGGTAAGAGAAAAAAAAACCTG
AATATTTAAGATCTTTACACACTCTCCATTTAACATCTTTGGTTATGGATTCTTATGTGA
ATAAATTAATAAGGAATATCTCAATCTGTTTTATGATGTTTGTTTCTTGATGCAGCACAT
ACCGGCTCATGTCATAAGGGACTATGACTTAACCTTCCCGGAGCGTATAACAGTCGTGGA
CGAAATGGGAGCATTGGAGAAAGCAATCAAGATTCAAAAGAATGGTTGCATCTTTGTCAA
GGGATTTGGTAGTTTCATCAGAAGGAACAAAATGAAGATGACTGACAAAATGATATGC

Last Updated on 06.12.2018 16:11