Sequences from confirmation amplicon


Confirmation sequences for line 037G08 and hit At5g04970 are shown.
Line ID 037G08
Locus At5g04970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1466732
Primers used for PCR 08nj - 8474, show primer details
   
Sequence Name 09-K114479-0022-037-G08C-8409
GenBank Accession LN490285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114479-0022-037-G08C-8409
TCACTCCTTACCCAAGCTCTGCCGCACGATCCTCTCCGCCGTTAAATCATCACCTTCCGA
TCCTTACCATTACGGAAAGTTCACAATGAAGCAATGCCTCAAACAAGCTCGTCGCCTCTC
CAAAGTCATCAACCGCTTTGCCCAGCGCGTGGAAGCTGATCCAGGTACGTCGACGGTGGA
AGAGGTCAGTGCGGTGGCTGATTGCGGTGAGCTAGCAGAGCTTAGCGTCGAATACCTTGA
AACGGTTACGGAGGAGCTGAAAGCGGCGGAGCTGATGACGGCTGCGCTCGTTGATCGTGT
CACGAGTCTTCTCGGTGGCGTTGTGACTAATCAGCAGACGTGCCTCGACGGTCTTGTGGA
CGCTAAGAGCGGATTTGCGACGGCGATTGGAACGCCGTTAGGGAATCTAACGCGCCTCTA
CAGTGTGTCGTTGGGGCTTGTGAGTCACGCGCTTAACCGTAATTTGAAGAGGTATAAAGT
TTCCAAGGG
   
Sequence Name 25-K114480-0022-037-G08C-08nj
GenBank Accession LN497555 [GenBank]
Sequencing primer gene specific primer, 08nj: 5'-CCCTTGGAACCTTTATACCTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K114480-0022-037-G08C-08nj
AGAGGCGCGTTAGATTCCCTAACGGCGTTCCAATCGCCGTCGCAAATCCGCTCTTAGCGT
CCACAAGACCGTCGAGGCACGTCTGCTGATTAGTCACAACGCCACCGAGAAGACTCGTGA
CACGATCAACGAGCGCAGCCGTCATCAGCTCCGCCGCTTTCAGCTCCTCCGTAACCGTTT
CAAGGTATTCGACGCTAAGCTCTGCTAGCTCACCGCAATCAGCCACCGCACTGACCTCTT
CCACCGTCGACGTACCTGGATCAGCTTCCACGCGCTGGGCAAAGCGGTTGATGACTTTGG
AGAGGCGACGAGCTTGTTTGAGGCATTGCTTCATTGTGAACTTTCCGTAATGGTAAGGAT
CGGAAGGTGATGATTTAACGGCGGAGAGGATCGTGCGGCAGAGCTTTGGGTAAGGAGTGG
ATTTACAAGCGATGTTTGGTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT

Last Updated on 06.12.2018 16:11