Sequences from confirmation amplicon


Confirmation sequences for line 038B01 and hit At5g05690 are shown.
Line ID 038B01
Locus At5g05690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1705186
Primers used for PCR MS63 - 8474, show primer details
   
Sequence Name 37-K017120-022-038-B01C-MS63
GenBank Accession LN502430 [GenBank]
Sequencing primer gene specific primer, MS63: 5'-AAAATCTAGACACCCGTGTGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K017120-022-038-B01C-MS63
TAAATGATGGAGAAATTTAGAGGCCAACATGCACATATCATTATGCTCCCATTGTTGTAG
TCTCTGTCCCATTTCTTTCTTTCACCTTAAATTTTATTTTTCCCCCAACTACTTCTTCCC
ATCTCTTAATTACTTAAGAAAATGAAATAAGCTTAAATCAGTAAGATTGTCCCCACAAGA
AAAAGTTCATATTAGTTGATAACAACACATCACCGTGTTCTAATTCAATGTTTTCCACTC
TTTTCGTTATTTTGTTGAAAGTACAGAAATGTAATTTAATTAGTTTTTCAAAGAAAAGAA
ACAAAACCATTTTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 38-K017120-022-038-B01C-8409
GenBank Accession LN502809 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K017120-022-038-B01C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAAAATGGTTTTGTT
TCTTTTCTTTGAAAAACTAATTAAATTACATTTCTGTACTTTCAACAAAATAACGAAAAG
AGTGGAAAACATTGAATTAGAACACGGTGATGTGTTGTTATCAACTAATATGAACTTTTT
CTTGTGGGGACAATCTTACTGATTTAAGCTTATTTCATTTTCTTAAGTAATTAAGAGATG
GGAAGAAGTAGTTGGGGGAAAAATAAAATTTAAGGTGAAAGAAAGAAATGGGACAGAGAC
TACAACAATGGGAGCATAATGATATGTGCATGTTGGCCTCTAAATTTCTCCATCATTTAC
GTTTCACACGGGTGTC
Primers used for PCR FL77 - 8474, show primer details
   
Sequence Name 11-K029742-022-038-B01C-8409
GenBank Accession LN490983 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K029742-022-038-B01C-8409
ATTGAATATATTTAAAACATGCGCCGGTGGTCCTTCAAAAAAACGGTTTGCATGCAGATG
CAATGTAGTGAACACTGAATAGATCGAATCTTAATTTTTTAATATCTTTTCCAAAACACT
AAATCTTGTTGATTCGCAATCAAACCAACTACATAACACCTAAAATTTCATTCATACGTG
TCGTGTTTTTCATTCGCTGTGTATACTCTTTTAAAAATAATTCAATCAAAATTCGATACT
AAAATCGAATATTTTTCTCAAAGTTATTCTTTATTAATTAAATACGAACCATGTATATAA
ATAAGTAGAGAGATTATTTACCAAAAATGGGAAAATTACCTTGAGTTGAGCAAGAGCTAA
AGGAGTCTCGGTGAGAAATTTGACGGCGAGAGTCATGATCGTGGAGGTTGTTTCATAACC
GGCGACAAGTAAAGCC
   
Sequence Name 12-K029742-022-038-B01C-FL77
GenBank Accession LN491443 [GenBank]
Sequencing primer gene specific primer, FL77: 5'-GACTTCTTGGTGGCTTTACTTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K029742-022-038-B01C-FL77
GATCATGACTCTCGCCGTCAAATTTCTCACCGAGACTCCTTTAGCTCTTGCTCAACTCAA
GGTAATTTTCCCATTTTTGGTAAATAATCTCTCTACTTATTTATATACATGGTTCGTATT
TAATTAATAAAGAATAACTTTGAGAAAAATATTCGATTTTAGTATCGAATTTTGATTGAA
TTATTTTTAAAAGAGTATACACAGCGAATGAAAAACACGACACGTATGAATGAAATTTTA
GGTGTTATGTAGTTGGTTTGATTGCGAATCAACAAGATTTAGTGTTTTGGAAAAGATATT
AAAAAATTAAGATTCGATCTATTCAGTGTTCACTACATTGCATCTGCATGCAAACCGTTT
TTTTGAAGGACCACCGGCGCATGTTTTAAATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11