Genotyping and primer details


Genotyping and primer details for 038B01 - At5g05690
Line ID 038B01
Locus At5g05690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1705186 of line 038B01 Chr5:1705186
   
Insertion allele amplicon 8474 - FL77
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL77: 5'-GACTTCTTGGTGGCTTTACTTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1705620
   
Insertion allele amplicon 8474 - MS63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MS63: 5'-AAAATCTAGACACCCGTGTGAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1704808
   
wt amplicon (over InsPos) FL77 - MS63
wt amplicon start Chr5:1704808
wt amplicon end Chr5:1705620
wt amplicon size 813 bp
Locus specific primer MS63: 5'-AAAATCTAGACACCCGTGTGAAAC-3'
2nd locus specific primer FL77: 5'-GACTTCTTGGTGGCTTTACTTGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11