Sequences from confirmation amplicon


Confirmation sequences for line 038B11 and hit At2g44460 are shown.
Line ID 038B11
Locus At2g44460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18348795
Primers used for PCR VL07 - 8474, show primer details
   
Sequence Name 41-K017367-022-038-B11C-VL07
GenBank Accession LN503894 [GenBank]
Sequencing primer gene specific primer, VL07: 5'-AATTTGTGTGACAGACTCAAGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K017367-022-038-B11C-vl07
ACCTTACAACTCTGCTTCTCCTGCCGACAACGAAGCTGTTAAACGAGCTCTTGCCACTGA
ACTAGATTGGTATAAGCTGTTCTATACTTTAAATAAGCAATGTTATCTTTATAGTAATCA
TAAGATATTTATTTATATTTTTCCAATGTGTGTATCAGGCATTTAGATCCAGTTATTCAC
GGAGATTATCCAGAAATGATGAAGAAACTTGCCGGAAATAGATTACCTTCGTTCACTCCA
GAACAATCCAAAATGTTGAAAAATTCATCGGATTTCATTGGAATAAACTACTATACAGCG
CGTTACGTTGCTCATATTCCACAAGCCGATCCTGCTCGTCCGCGATTCGTAACCGATCAT
CAGCTCCAATGGCGAGGTAAGATTGCTAATGTTAACATCCGGACAATATGGCCATTTACA
TGAAGCCATCATACTCCAGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCATTCAAAA
   
Sequence Name 45-K017367-022-038-B11C-8409
GenBank Accession LN505361 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K017367-022-038-B11C-8409
CAATTGAATATATCCTGATCTGAGTATGATGGCTTCATGTAAATGGCCATATTGTCCGGA
TGTTAACATTAGCAATCTTACCTCGCCATTGGAGCTGATGATCGGTTACGAATCGCGGAC
GAGCAGGATCGGCTTGCGGAATATGAGCAACGTAACGCGCTGTATAGTAGTTTATTCCAA
TGAAATCCGATGAATTTTTCAACATTTTGGATTGTTCTGGAGTGAACGAAGGTAATCTAT
TTCCGGCAAGTTTCTTCATCATTTCTGGATAATCTCCGTGAATAACTGGATCTAAATGCC
TGATACACACATTCCAAAAATATAAATAAATATCTTATGATTACTATAAGGATAACATTA
CTTATCTAAAGAATATAACAACTTATACCAATCTAGTTCAGTGGCAAGAGCTCGTTTAAC
AGCTTCGTTGTCGGCAGGAGAAG
Primers used for PCR FL78 - 8474, show primer details
   
Sequence Name 03-K035332-022-038-B11C-8409
GenBank Accession LN487383 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K035332-022-038-B11C-8409
TTCCGGACATGAGCCATCATACTCTTTGTATGAGATTCAATAAAATTCCTAAACTAGTTT
TTTTTGTCTGTTTATGAACAGTAACAAATCACAGTAATCACCAATTCGGACCAGGGGTAC
GTAACATTTTTGACTAGTTTTGTTACAAAATCAACGTTGCAATTATTTTTACTTTTTATT
AATTGATCCAAAGATTCTAATAAGAAGATAAATGCTTGTTTTTGAAAAACAGGAAGATCG
AGGCATACTTCAATCACATCCTGAAGGTTTACGAAAAGTTCTTAACTACATCAAAGATAA
ATACAACAACCCTATAGTTTATATCAAAGAGAACGGTAAGAATATTTTACGAATTTGTTT
TTTTTAATCTATTAATTAAACAGTTTCCTCTCTTTGTTAATTGCCAAAATATTTTTATAG
GAATTAATGACTACGACGACGGTACAAAATCACGAGAGGAGATTCTCAATGATACATTTA
GGATAAGCTACCATGAGGACCATCTCC
   
Sequence Name 03-K035332-022-038-B11C-FL78
GenBank Accession LN487384 [GenBank]
Sequencing primer gene specific primer, FL78: 5'-ATAGCTTTTTGGAGTTGTTGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K035332-022-038-B11C-FL78
ATGTATCATTGAGAATCTCCTCTCGTGATTTTGTACCGTCGTCGTAGTCATTAATTCCTA
TAAAAATATTTTGGCAATTAACAAAGAGAGGAAACTGTTTAATTAATAGATTAAAAAAAA
CAAATTCGTAAAATATTCTTACCGTTCTCTTTGATATAAACTATAGGGTTGTTGTATTTA
TCTTTGATGTAGTTAAGAACTTTTCGTAAACCTTCAGGATGTGATTGAAGTATGCCTCGA
TCTTCCTGTTTTTCAAAAACAAGCATTTATCTTCTTATTAGAATCTTTGGATCAATTAAT
AAAAAGTAAAAATAATTGCAACGTTGATTTTGTAACAAAACTAGTCAAAAATGTTACGTA
CCCCTGGTCCGAATTGGTGATTACTGTGATTTGTTACTGTTCATAAACAGACAAAAAAAA
CTAGTTTAGGAATTTTATTGAATCTCATACAAAGAGTATGATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
Primers used for PCR FL78 - 8474, show primer details
   
Sequence Name 41-K118471-0022-038-B11C-FL78
GenBank Accession LN504259 [GenBank]
Sequencing primer gene specific primer, FL78: 5'-ATAGCTTTTTGGAGTTGTTGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118471-0022-038-B11C-FL78
GAGAATCTCCTCTCGTGATTTTGTACCGTCGTCGTAGTCATTAATTCCTATAAAAATATT
TTGGCAATTAACAAAGAGAGGAAACTGTTTAATTAATAGATTAAAAAAAACAAATTCGTA
AAATATTCTTACCGTTCTCTTTGATATAAACTATAGGGTTGTTGTATTTATCTTTGATGT
AGTTAAGAACTTTTCGTAAACCTTCAGGATGTGATTGAAGTATGCCTCGATCTTCCTGTT
TTTCAAAAACAAGCATTTATCTTCTTATTAGAATCTTTGGATCAATTAATAAAAAGTAAA
AATAATTGCAACGTTGATTTTGTAACAAAACTAGTCAAAAATGTTACGTACCCCTGGTCC
GAATTGGTGATTACTGTGATTTGTTACTGTTCATAAACAGACAAAAAAAACTAGTTTAGG
AATTTTATTGAATCTCATACAAAGAGTATGATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTC
   
Sequence Name 09-K118470-0022-038-B11C-8409
GenBank Accession LN490401 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118470-0022-038-B11C-8409
ACTCTTTGTTGAGATTCAATAAAATTCCTAAACTAGTTTTTTTCGTCTGTTTATGAACAG
TAACAAATCACAGTAATCACCAATTCGGACCAGGGGTACGTAACATTTTTGACTAGTTTT
GTTACAAAATCAACGTTGCAATTATTTTTACTTTTTATTAATTGATCCAAAGATTCTAAT
AAGAAGATAAATGCTTGTTTTTGAAAAACAGGAAGATCGAGGCATACTTCAATCACATCC
TGAAGGTTTACGAAAAGTTCTTAACTACATCAAAGATAAATACAACAACCCTATAGTTTA
TATCAAAGAGAACGGTAAGAATATTTTACGAATTTGTTTTTTTTAATCTATTAATTAAAC
AGTTTCCTCTCTTTGTTAATTGCCAAAATATTTTTATAGGAATTAATGACTACGACGACG
GTACAAAATCACGAGAGGAGATTCTCAATGATACATTTAGGATAAGCTACCATGAGGACC
ATCTCCAACAACTCAAAAAAAGCTAT

Last Updated on 06.12.2018 16:11