Sequences from confirmation amplicon


Confirmation sequences for line 038D05 and hit At1g16930 are shown.
Line ID 038D05
Locus At1g16930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5790543
Primers used for PCR 36mm - 8474, show primer details
   
Sequence Name 50-K114263-0022-038-D05C-36mm
GenBank Accession LN507248 [GenBank]
Sequencing primer gene specific primer, 36mm: 5'-CACTGAGAAAATGTGATAAAGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K114263-0022-038-D05C-36mm
GATCGAGCATATATATACATGTAGAGTATCAGAAGATATGACCATTTCGCTGACCGTAGA
TAACCCAGTGAGGAATTTACCTAGCATAGCTATCTTGGAAGAATCATCTGGTTCCAATGG
ATCATTATATTCGACATTAAAGATAACATCGATATCTACCTTTGCAAAGGGGCCAATGTT
GTGTATTATGAAGCTGTCAGATCGATGATCACAAAGATTCATATACTCAAGTCTTGGAGC
ATCGATTGTAACAACATGATTTTGACTTTCGTAGCGCTCAGAATCTATCTTGAAGCTTTT
TAAAGACTGGGAACGCACACACACAACTTCAAGAGAATCATTGGGATCACGAACTATGGT
TAACTCTTCCAAAACTGGACAACTACATGCACAAGTAGATGGACAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCACAAATG
   
Sequence Name 07-K114262-0022-038-D05C-8409
GenBank Accession LN489369 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114262-0022-038-D05C-8409
TATATCCTGTCCATCTACTTGTGCATGTAGTTGTCCAGTTTTGGAAGAGTTAACCATAGT
TCGTGATCCCAATGATTCTCTTGAAGTTGTGTGTGTGCGTTCCCAGTCTTTAAAAAGCTT
CAAGATAGATTCTGAGCGCTACGAAAGTCAAAATCATGTTGTTACAATCGATGCTCCAAG
ACTTGAGTATATGAATCTTTGTGATCATCGATCTGACAGCTTCATAATACACAACATTGG
CCCCTTTGCAAAGGTAGATATCGATGTTATCTTTAATGTCGAATATAATGATCCATTGGA
ACCAGATGATTCTTCCAAGATAGCTATGCTAGGTAAATTCCTCACTGGGTTATCTACGGT
CAGCGAAATGGTCATATCTTCTGATACTCTACAGGTATATATATGCTCGATTCAATAACA
TTAAGCTTATACACTTCCTTTATGGCTTTATCACATTTTCTCAGT

Last Updated on 06.12.2018 16:11