Sequences from confirmation amplicon


Confirmation sequences for line 038F09 and hit At5g58660 are shown.
Line ID 038F09
Locus At5g58660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23702164
Primers used for PCR KR30 - 8474, show primer details
   
Sequence Name 16-K030753-022-038-F09C-8409
GenBank Accession LN493316 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K030753-022-038-F09C-8409
TATAGTTTTTGTCTTTAGTATATAGTCCATGTGTACAGCAGCTAGTTACACCTATATTTT
AATTATTGAGTACTTTAACGTCGATTGATTGATTAATTAATCGCGGAAACCACTAATGAC
ACTATTAAGTATTAAGCAACGAAGGTAAACGAAAACGACGACGTATGTTACCTGAAAGAC
TCAAGTTTAGGCTCTTCTTGAGCATCATCATCACAACAAGTAGAAGTTGGAAGCTTCGAG
AGCGACGAGAGAGACGAGAGAGGAACGTTGAAACCTTCGAGCATGGTTAAGTTCGAAGCT
TGAGCTCCTCTCTTTAGTGCATCTCCTGAACGATTTAGAGCTGGTGTTCCCCAAAAATAC
GATAACGGAGATTTAACGGCAGCGAACAATTCCCGTTTTTTCTCAAACGGAAGACTCAGT
AATGACTCCGAGATTTCTTGAAGCCGTGACGTTAACGCCAACGGTACTCCGTGATTCTCT
AG
   
Sequence Name 55-K030753-022-038-F09C-KR30
GenBank Accession LN508705 [GenBank]
Sequencing primer gene specific primer, KR30: 5'-TCTAGAGAATCACGGAGTACCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K030753-022-038-F09C-KR30
TTACTGAGTCTTCCGTTTGAGAAAAAACGGGAATTGTTCGCTGCCGTTAAATCTCCGTTA
TCGTATTTTTGGGGAACACCAGCTCTAAATCGTTCAGGAGATGCACTAAAGAGAGGAGCT
CAAGCTTCGAACTTAACCATGCTCGAAGGTTTCAACGTTCCTCTCTCGTCTCTCTCGTCG
CTCTCGAAGCTTCCAACTTCTACTTGTTGTGATGATGATGCTCAAGAAGAGCCTAAACTT
GAGTCTTTCAGGTAACATACGTCGTCGTTTTCGTTTACCTTCGTTGCTTAATACTTAATA
GTGTCATTAGTGGTTTCCGCGATTAATTAATCAATCAATCGACGTTAAAGTACTCAATAA
TTAAAATATAGGTGTAACTAGCTGCTGTACACATGGACTATATACTAAAGACAAAAACTA
TATGGACTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11