Sequences from confirmation amplicon


Confirmation sequences for line 038G01 and hit At1g34180 are shown.
Line ID 038G01
Locus At1g34180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12449624
Primers used for PCR 01yv - 8474, show primer details
   
Sequence Name 19-K122558-0022-038-G01C-01yv
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 01yv: 5'-TTGGTTTTCTAACACGGTGAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K122558-0022-038-G01C-01yv
GTTCTTTTTACTCCAGGAATAGGAAGTATCCTAACGCAGCTAGGTCAAGTAGAGGTACTG
CAACTGGTTATTGGAAGGCGACAGGAAAGGATCGAGTCATTGAGTACAATTCAAGATCTG
TTGGACTCAAGAAGACTCTTGTTTTCTATAGAGGTCGTGCTCCTAATGGTGAGAGAACTG
ACTGGGTGATGCATGAGTACACTATGGATGAAGAAGAGCTAGGGAGATGTAAGAACGCTA
AGGTTAGTATTATAGCCAAAACTCATCTGTTTCTCTTTTTGATTTATTTGTAGTTTAACT
AATCTGTCTTACCTTCAGGAGTATTATGCTCTTTATAAGCTTTATAAGAAGAGTGGGGCT
GGTCCTAAGAATGGTGAACAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCACAGCGTTATTA
   
Sequence Name 03-K122557-0022-038-G01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K122557-0022-038-G01C-8409
TTGAATTGTTCACCATTCTTAGGACCAGCCCCACTCTTCTTATAAAGCTTATAAAGAGCA
TAATACTCCTGAAGGTAAGACAGATTAGTTAAACTACAAATAAATCAAAAAGAGAAACAG
ATGAGTTTTGGCTATAATACTAACCTTAGCGTTCTTACATCTCCCTAGCTCTTCTTCATC
CATAGTGTACTCATGCATCACCCAGTCAGTTCTCTCACCATTAGGAGCACGACCTCTATA
GAAAACAAGAGTCTTCTTGAGTCCAACAGATCTTGAATTGTACTCAATGACTCGATCCTT
TCCTGTCGCCTTCCAATAACCAGTTGCAGTACCTCTACTTGACCTAGCTGCGTTAGGATA
CTTCCTATTCCTTGGAGTGAAAAAGAACCACTGTCTATCTCCCGTCTTCAACATCGATAG
ACCTGTTACATTCACCGTGTTAGAAAACCA

Last Updated on 06.12.2018 16:11